Overview
DNAbsolute is a high yield and plastic-free way to purify your genomic DNA. Based on ionic liquids, DNAbsolute does not involve any plastic column.
A technology designed by Guillaume Barnoin, Nicolas Papaiconomou, Benoît Michel, Nadine Martinet, Alain Burger.
"We dreamed of an alternative to purify our DNA that would be both safer to the Phenol-Chloroform method and greener than the plastic column-based methods."Guillaume Barnoin, Nicolas Papaiconomou, Benoît Michel, Nadine Martinet, Alain Burger
Images
Features
High yield
~85% recovery with a range of 10 to 100 µg/mL
~60% recovery with low DNA concentrations, such as 5-10 ng/mL
High purity of the recovered DNA
260 / 280 ratio = 1,8 - 1,9
260 / 230 ratio = 2,3
Even for low concentrations
Purify DNA as low as 5 ng/mL
The pellet is visible to the naked eye from 1 µg/mL of DNA
Plastic-free
No column involved, just ionic liquids
Protocol
Technical information
Read the Safety Datasheet.
Read the most Frequently Asked Questions.
DNAbsolute exists in 3 volumes: 5 mL, 25 mL and 50 mL.
The 5mL kit includes:
- 5 mL of DNAbsolute 3M
- 5 mL of PBS 150 mM, pH 7.2
- 5 mL vial of a Tris solution, 25 mM, pH 8
- 12 mL of Lysis buffer 1*
- 12 mL of Lysis buffer 2*
It allows 25 experiments with fruit flies, with the protocol included in the kit.
*The lysis buffer included in the DNAbsolute kit is compatible with a wide range of sample types (blood, tissues, cells, saliva, soil, whole organisms, etc). If needed, your own lysis buffer can be used instead of the one provided in the kit.
Story
Guillaume Nicolas Benoît Nadine Alain
Esther Graudens,
New Projects manager at Idylle, with Nicolas, Benoît and Alain