Overview

DNAbsolute is a high yield and plastic-free way to purify your genomic DNA. Based on ionic liquids, DNAbsolute does not involve any plastic column.
A technology designed by Guillaume Barnoin, Nicolas Papaiconomou, Benoît Michel, Nadine Martinet, Alain Burger.
"We dreamed of an alternative to purify our DNA that would be both safer to the Phenol-Chloroform method and greener than the plastic column-based methods."Guillaume Barnoin, Nicolas Papaiconomou, Benoît Michel, Nadine Martinet, Alain Burger
Images
Features

High yield
~85% recovery with a range of 10 to 100 µg/mL
~60% recovery with low DNA concentrations, such as 5-10 ng/mL

High purity of the recovered DNA
260 / 280 ratio = 1,8 - 1,9
260 / 230 ratio = 2,3

Even for low concentrations
Purify DNA as low as 5 ng/mL
The pellet is visible to the naked eye from 1 µg/mL of DNA

Plastic-free
No column involved, just ionic liquids
Protocol
Technical information

Read the Safety Datasheet.
Read the most Frequently Asked Questions.
DNAbsolute exists in 3 volumes: 5 mL, 25 mL and 50 mL.
The 5mL kit includes:
- 5 mL of DNAbsolute 3M
- 5 mL of PBS 150 mM, pH 7.2
- 5 mL vial of a Tris solution, 25 mM, pH 8
- 12 mL of Lysis buffer 1*
- 12 mL of Lysis buffer 2*
It allows 25 experiments with fruit flies, with the protocol included in the kit.
*The lysis buffer included in the DNAbsolute kit is compatible with a wide range of sample types (blood, tissues, cells, saliva, soil, whole organisms, etc). If needed, your own lysis buffer can be used instead of the one provided in the kit.
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