DNAbsolute
DNAbsolute is a high yield and plastic-free way to purify your genomic DNA. Based on ionic liquids, DNAbsolute does not involve any plastic column.
We dreamed of an alternative to purify our DNA that would be both safer to the Phenol-Chloroform method and greener than the plastic column-based methods.
The Test Program is open!
Registrations are closing on: February 27, 2023
The objectives of this 1st campaign are to test your own applications of DNAbsolute.
Amongst those, we would mostly value tests with:
Circulating DNA
Plant cells
Plasma and serum samples.
Number of days before the registrations close:
You are new to the Test Programs by Idylle? Discover them here!
Features
High yield
~85% recovery with a range of 10 to 100 µg/mL
~60% recovery with low DNA concentrations, such as 5-10 ng/mL
High purity of the recovered DNA
260 / 280 ratio = 1,8 - 1,9
260 / 230 ratio = 2,3
Even for low concentrations
Purify DNA as low as 5 ng/mL
The pellet is visible to the naked eye from 1 µg/mL of DNA
Plastic-free
No column involved, just ionic liquids
Gallery
Simplified protocol
Product information
DNAbsolute exists in 3 volumes: 5 mL, 25 mL and 50 mL.
The 5mL kit includes:
- 5 mL of DNAbsolute 3M
- 5 mL of PBS 150 mM, pH 7.2
- 5 mL vial of a Tris solution, 25 mM, pH 8
- 12 mL of Lysis buffer 1
- 12 mL of Lysis buffer 2
It allows 25 experiments with fruit flies, with the protocol included in the kit.
DNAbsolute and us
Guillaume Barnoin, Nicolas Papaiconomou, Benoît Michel, Nadine Martinet, Alain Burger
Guillaume Nicolas Benoît Nadine Alain
Esther Graudens,
New Projects manager at Idylle, with Nicolas, Benoît and Alain