
Helixohm
Helixohm probes are extremely stable electroactive compounds used as molecular probes for the detection of nucleic acids in molecular biology. They consist in a range of probes with different standard electroactive potentials.
Designed by
Damien Marchal
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LEox technology
published in ACS Publications
How to use it? Check our simplified protocol
Why we chose it? Learn about the story of Helixohm and us
Features

Chemical Formula
[Os(bpy)2dppz] (PF6)2

PM
1074,83 M

E°
0,65 V/SCE

Kb(25°C) / Kb(60°C)
1 500 000 M-1 / 400 000 M-1

Robust & reliable
LEox technology can be used in a turbid environment and raw samples (like saliva, urine, sweat, etc.) and in the presence of fluorescence inhibitors

Versatility
Compatible with a large range of applications like genotyping, SNP detection, normal shift assay, conventional biosensing on DNA, RNA and protein samples

Activity
Electroactive but no fluorescent properties reported to date

Techniques
PCR reactionLAMP reaction Conventional electrochemical measurement
Applications
Combined with in-vitro gene amplification, LEO probes allow indirect electrochemical detection of DNA at subattomolar concentrations - a few DNA - copies. Combined with a melting curve, LEO probes allow to differentiate two different amplified DNA strands by a single nucleic base pair. Since the LEO probe is an electroactive molecule, its concentration, free in solution or bound to double-stranded DNA, is measured by an electrochemical measurement, usually square wave voltammetry.
Gallery
Protocol
Helixohm by Damien Marchal
Contact Damien Marchal about Helixohm.