Pept'it

Safely deliver nucleic acids in your favorite cells!

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What is it for?  Use it if you want to transfect cells with siRNA.  

Designed by  Prisca Boisguerin, Gudrun Aldrian, Karidia Konate, Eric Vivès, Sébastien Deshayes.

Powered by
the WRAP technology published in Bioconjugate Chemistry.

Features

Easy to handle

Mix Pept'it and siRNA to form nanoparticles and transfect your cells

 


Direct cell translocation

Nanoparticles enter the cells through a non-endocytic pathway

Rapid effect

The inhibition of gene expression is visible after 30 minutes only

Safe for cells

No toxic side effect compared to standard transfection reagents

Gallery

Animated protocol

 
 
                                                                                                                 

Simplified protocol

Odoo - Sample 1 for three columns
Odoo - Sample 2 for three columns
Odoo - Sample 3 for three columns

WRAP, Prisca, Gudrun, Karidia, Eric, Sébastien and us

We are a group of five researchers coming from different scientific fields (biology, biochemistry, chemistry and biophysics) but what we have in common is the development of cell-penetrating peptides as a delivery system for therapeutic molecules. However, because most of the common delivery systems are internalized via endocytosis-dependent pathways (= therapeutic molecules with weak accessibility), we decided to put all our common knowledge on cell-penetrating peptides together to design new peptides for the cellular delivery of nucleic acids via direct cell translocation (= therapeutic molecules with fast accessibility). After a short screening using a standard procedure, the WRAP (W- and R-rich peptide) family was selected as effective siRNA delivery systems without any toxic side-effects compared to other transfection reagents. 
Interdisciplinarity and complementary expertise of our consortium seem to be the accurate ingredients for a successful “recipe”.
Odoo • Image and Text
Eric, Gudrun, Prisca, Karidia, Sébastien
Getting a hard time transfecting your favorite cell line or inhibiting your favorite gene? 

Bibliography

Deshayes S, Konate K, Vivès E, Boisguérin P. Tips and Tools to Understand Direct Membrane Translocation of siRNA-Loaded WRAP-Based Nanoparticles. Methods Mol Biol. 2022;2383:475-490. doi: 10.1007/978-1-0716-1752-6_30

Konate K, Josse E, Tasic M, Redjatti K, Aldrian G, Deshayes S, Boisguérin P, Vivès E. WRAP-based nanoparticles for siRNA delivery: a SAR study and a comparison with lipid-based transfection reagents. J Nanobiotechnology. 2021 Aug 11;19(1):236. doi: 10.1186/s12951-021-00972-8.

Faria R, Vivés E, Boisguerin P, Sousa A, Costa D. Development of Peptide-Based Nanoparticles for Mitochondrial Plasmid DNA Delivery. Polymers (Basel). 2021 Jun 1;13(11):1836. doi: 10.3390/polym13111836.

Boisguérin P, Konate K, Josse E, Vivès E, Deshayes S. Peptide-Based Nanoparticles for Therapeutic Nucleic Acid Delivery. Biomedicines. 2021 May 20;9(5):583. doi: 10.3390/biomedicines9050583. Review.

Ferreiro I, Genevois C, Konate K, Vivès E, Boisguérin P, Deshayes S, Couillaud F. In Vivo Follow-Up of Gene Inhibition in Solid Tumors Using Peptide-Based Nanoparticles for siRNA Delivery. Pharmaceutics. 2021 May 19;13(5):749. doi: 10.3390/pharmaceutics13050749.

 Konate K, Seisel Q, Vivès E, Boisguérin P, Deshayes S. Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide. J Vis Exp. 2020 Dec 19;(166). doi: 10.3791/62028.

Deshayes S, Konate K, Dussot M, Chavey B, Vaissière A, Van TNN, Aldrian G, Padari K, Pooga M, Vivès E, Boisguérin P. Deciphering the internalization mechanism of WRAP:siRNA nanoparticles. Biochim Biophys Acta Biomembr. 2020 Jun 1;1862(6):183252. doi: 10.1016/j.bbamem.2020.183252.

Sousa Â, Almeida AM, Faria R, Konate K, Boisguerin P, Queiroz JA, Costa D. Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments. Colloids Surf B Biointerfaces. 2019 Nov 1;183:110417. doi: 10.1016/j.colsurfb.2019.110417.

Konate K, Dussot M, Aldrian G, Vaissière A, Viguier V, Neira IF, Couillaud F, Vivès E, Boisguerin P, Deshayes S. Peptide-Based Nanoparticles to Rapidly and Efficiently "Wrap 'n Roll" siRNA into Cells. Bioconjug Chem. 2019 Mar 20;30(3):592-603. doi: 10.1021/acs.bioconjchem.8b00776.