The phase-only diffraction grating designed for optimal super-resolution PSF sampling. Phimask allows 3D localization deep in tissue with a quasi-isotropic precision and limited photon loss (<20%).
Features Protocol Story
Adapted to any excitation modality
Phimask is inserted between the microscope image plane and the camera, in the emission path
Compatible with multiple colors
Phimask is compatible with up to 3 colors by default in the 500 – 750 nm range
Quasi-isotropic single-shot 3D localization
Extract the 3D localization of a single particle in 1 frame, with a quasi-isotropic precision
Very limited photon loss
Phimask is capable of generating interferences with limited photon loss (<20%)
A non degraded lateral 2D resolution
Since the PSF has very limited broadening and almost all photons contribute to the image
Compatible with any optical microscopy technique
as long as it is capable of providing single fluorescent objects (dSTORM,PALM, single particle imaging, uPAINT)
May 7, 2020. Pierre Bon shared his insights on Phimask before answering scientific questions from his research community. We also learnt about the best implementation of Phimask on a microscope, as well as the algorithm to extract the 3D position of each emitter. A great moment sharing scientific know-how together!
Installation & image analysis protocol
Phimask, Pierre Bon and us
Contact Pierre Bon about his technology.
New projects at Idylle
"Our relationships with Pierre started even before Idylle was born! We were closely following his work and his papers. When we started our operations, it was just natural to invite him to get onboard. He kindly replied with enthusiasm and our story with Phimask began.
Since then, we have lived a few premieres together. We were lucky that Pierre is that kind of man who says yes first. We share the same love for new (sometimes crazy?) ideas. An example? He immediately jumped on our webinar proposal - and therefore Pierre stands as our first ever talk guest!"
Contact us if you want to know more about the best way to set up Phimask on your microscope - or discuss your research project.