powered by the SELFI technology
"I have discovered Phimask by chance ! (as usual?)...
By the end of my PhD I was adjusting in a very unconventional manner a grating-based interferometer normally adapted to white-light microscopy. I observed fringes when imaging a incoherent light source, where theoretically I shouldn't have observed anything. This made me realize that the interferometer could be adapted to fluorescence sources and 6 years after it was working on a single molecule based fluorescent microscope!"
EXAMPLES OF RESULTS
The phase-only diffraction grating designed for optimal super-resolution PSF sampling. Phimask allows 3D localization deep in tissue with a quasi-isotropic precision and limited photon loss (<20%).
Image credit: Pierre Bon 2020
Image credit: Pierre Bon, Jeanne Linarès-Loyez 2020.
Image credit: Pierre Bon, Jeanne Linarès-Loyez, Grégory Giannone 2020.
Adapted to any microscope: The Phimask is inserted between the microscope image plane and the camera. It is thus compatible with any kind of fluorescent microscope.
Quasi-isotropic single-shot 3D localization: In one frame, one can extract the 3D localization of a single particle with a quasi-isotropic precision.
Very limited photon loss: Phimask is capable of generating interferences with limited photon loss (<20%).
A non degraded lateral 2D resolution: Since the PSF has very limited broadening and almost all photons contribute to the image, the lateral resolution is not degraded.