Odoo • Image et Texte

Phimask
The phase-only diffraction grating designed for optimal super-resolution PSF sampling. Phimask allows 3D localization deep in tissue with a quasi-isotropic precision and limited photon loss (<20%).


Designed by Pierre Bon

Published in Nature Methods


What is it for? Use it if turning your 2D SMLM single molecule localization microscopy images into 3D images is a fantasy for you.
How to install it? Check our installation & image analysis protocol
Why we chose it? Learn about the story of Phimask, Pierre Bon and us.

  Features

Adapted to any microscope

The Phimask is inserted between the microscope image plane and the camera. It is thus compatible with any kind of fluorescent microscope

Quasi-isotropic single-shot 3D localization

In one frame, one can extract the 3D localization of a single particle with a quasi-isotropic precision

Very limited photon loss

Phimask is capable of generating interferences with limited photon loss (<20%).

A non degraded lateral 2D resolution

Since the PSF has very limited broadening and almost all photons contribute to the image, the lateral resolution is not degraded

Compatibilities

Gallery

  Talk

May 7, 2020. Pierre Bon shared his insights on Phimask before answering scientific questions from his research community. We also learnt about the best implementation of Phimask on a microscope, as well as the algorithm to extract the 3D position of each emitter. A great moment sharing scientific know-how together!

 
 

  Installation & image analysis protocol

Phimask, Pierre Bon and us

"I have discovered Phimask by chance ! (as sual?)... By the end of my PhD I was adjusting  in a very unconventional manner a grating-based interferometer normally adapted to white-light microscopy. I observed fringes when imaging an incoherent light source, where theoretically I shouldn't have observed anything. This made me realize that the interferometer could be adapted to fluorescence sources and 6 years after it was working on a single molecule based fluorescent microscope!"

Contact Pierre Bon about his technology.

Odoo • Image and Text Esther Graudens
New projects at Idylle

"Our relationships with Pierre started even before Idylle was born! We were closely following his work and his papers. When we started our operations, it was just natural to invite him to get onboard. He kindly replied with enthusiasm and our story with Phimask began.
Since then, we have lived a few premieres together. We were lucky that Pierre is that kind of man who says yes first.
We share the same love for new (sometimes crazy?) ideas. An example? He immediately jumped on our webinar proposal - and therefore Pierre stands as our first ever talk guest!"

Contact us if you want to know more about the best way to set up Phimask on your microscope - or discuss your research project.