Odoo • Image et Texte

Phimask

powered by the SELFI technology 


Designed by Pierre Bon

"I have discovered Phimask by chance ! (as usual?)...
By the end of my PhD I was adjusting  in a very unconventional manner a grating-based interferometer normally adapted to white-light microscopy. I observed fringes when imaging a incoherent light source, where theoretically I shouldn't have observed anything. This made me realize that the interferometer could be adapted to fluorescence sources and 6 years after it was working on a single molecule based fluorescent microscope!"
 

  EXAMPLES OF RESULTS

The phase-only diffraction grating designed for optimal super-resolution PSF sampling. Phimask allows 3D localization deep in tissue with a quasi-isotropic precision and limited photon loss (<20%).

Odoo • Image et Texte
How to build a SELFI-based interferometer using Phimask for fluorescence super-localization? (Up) The optical setup and expected point spread function (PSF) with SELFI. (Down) SELFI-PSF evolution long the z axis.
Image credit: Pierre Bon 2020
Phimask used with dSTORM for 3D super-resolution imaging. 3D super-resolved reconstruction using Phimask combined with dSTORM. 60x NA=1.3, Phalloidin-A647, human fibroblasts (WI-38).
Image credit: Pierre Bon, Jeanne Linarès-Loyez 2020.
Odoo • Image et Texte
Phimask used with PALM for 3D super-resolution imaging. 3D super-resolved imaging using Phimask combined with PALM. 60x NA=1.3, mEOS2 fused with Paxillin or Talin-C, mouse embryonic fibroblasts (MEF).
Image credit: Pierre Bon, Jeanne Linarès-Loyez, Grégory Giannone 2020.

  APPLICATIONS

  TECHNOLOGY FEATURES

Adapted to any microscope: The Phimask is inserted between the microscope image plane and the camera. It is thus compatible with any kind of fluorescent microscope.

Quasi-isotropic single-shot 3D localization: In one frame, one can extract the 3D localization of a single particle with a quasi-isotropic precision.

Very limited photon loss: Phimask is capable of generating interferences with limited photon loss (<20%).

A non degraded lateral 2D resolution: Since the PSF has very limited broadening and almost all photons contribute to the image, the lateral resolution is not degraded.

  METHODS

  PUBLICATIONS