SpheroRuler is a suspension of 1 μM diameter spheres coated with a 647-fluorophore that blinks in dSTORM experiments. SpheroRuler gives you confidence in the performance of your super-resolution imaging process. It assesses the validity of your settings.
How do you know your imaging process is good with SpheroRuler? Your beads blink, you reconstruct a ring in 2D and a sphere in 3D experiments, and you check that you retrieve the 1 µm diameter.
Why is SpheroRuler a must-have? It is great for dSTORM calibration experiments because you can use it on your preferred support and buffer. And because you get confident with the new biological structure to image. SpheroRuler is also a simple shape easy to study when you are a dSTORM beginner!
The Test Program is open!
Registration is starting on: October 25, 2022
The 1st campaign is closing on: January 24, 2023
The objectives of this 1st campaign are to test your own applications of SpheroRuler. We are also interested if you want to test and mount SpheroRuler together with your biological samples on the same dish.
Among others, we would value tests with:
Other SMLM modalities than dSTORM
Various mounting buffers like Glox or Vectashield for instance
Other supports than a glass bottom dish: depression slides, 8-well dish ...
You are new to the Test Programs by Idylle? Discover them here!
It's a spherical shape
that's easy to reconstruct
Blinking fluorescent dyes
To perform super-resolution microscopy such as dSTORM
Monodisperse spherical 1 µm beads
Usable together with organic samples
Resuspended in an aqueous buffer
SpheroRuler's simplified protocol
SpheroRuler and us
Arnaud Favier, Karine Monier and Christophe Place
Arnaud Favier, Karine Monier, Christophe Place
New business, Idylle