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Stampwell

powered by the Mount(n) technology


Designed by Gaëlle Recher

"The Mount(n) technology is the results of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into an hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies...). The design of the wells prevents the object from moving when the plate or dish is moved, does not apply any mechanical constrain to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary."  

  EXAMPLES OF RESULTS

The first stamp to imprint arrays of wells in hydrogels and to mount 3D biological samples for parallelized microscopy experiments. Stampwell ensures the sample stillness, long-term medium-throughput and automatized multi-position image acquisition for a significant timesaving.

 
 
Imprinted well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux

 
 
Cell-filled alginate capsule in the well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux

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Imprinted wells
Filled with a single cell-loaded alginate capsules; corresponding labels imaged with a stereomicroscope and stitched. Gel pad is made of Agarose 2%. Bar: 2mm.
Image credit:
(c)Gaëlle Recher - Bordeaux
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Zebrafish larva
In rectangle shape wells - bar=1mm (projection of Z-stack - confocal microscope)
Image credit:
(c)Gaëlle Recher - Bordeaux
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Cancer cells co-culture
In alginate capsule - bar = 100um
Image credit:
(c)Gaëlle Recher, Simon Latour - Bordeaux
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hiPSCs cyst in alginate capsule
bar = 500um
Image credit:
(c)Gaëlle Recher, Maxime Feyeux - Bordeaux

  APPLICATIONS

  TECHNOLOGY FEATURES

Medium/high throughput: 70 objects can be loaded with one stamp

Automatized multi-position image acquisition: Stampwell provides a constraint-free but stable and parallelized immobilization of the samples allowing automatized multi-position image acquisition

Long-term imaging: Because the sample is not embedded in the agarose pad but just laying on the bottom of a V-shaped well, it can freely grow within the well, enabeling culture and image aquisition over days and weeks

  METHODS

  PUBLICATIONS