Stampwell
A family of 7 stamps designed to make culture and imaging
of
zebrafish embryos, medaka and organoids
as easy as cost-effective
REQUEST A QUOTE TEST THE NEW SHAPES
Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids/organoids as well as fish embryos or larvae up to 20 dpf. At the request of the scientific communities, we have also developed specific stamps for the cell aggregation.
Designed by
Gaëlle Recher
The U-shape is codesigned with Camille Douillet
Powered by the Mount(n) technology (UniverSlide) published in
Scientific Reports
It all started with Gaëlle Recher's need to image her 3D culture objects, read the
story of her tech transfer journey
Those little extras that make all the difference:
For stamps with more than 10 wells, alphanumeric markers make it possible to quickly identify 1 particular sample.
When you move your dish from the incubator to the microscope, the samples remain quietly in their wells (except the Stampwell made for aggregation)
Image your biological samples growing for several weeks in the same dish.
Campaign #3: the Zebrafish Test Program
The first 2 campaigns have provided feedback of use for the imaging and culture of organoïds and spheroïds (V-shapes).
We are now looking for feedback on the technology for zebrafish / medaka imaging, and are happy to provide a new campaign of Test Program.
Registrations are closing on March 24.
Number of days left before we close the registrations:
I REGISTER NOW TO THE TEST PROGRAM
If you are not familiar with the Test Programs,
read more here
.
Overal feedback from the Test Program
Feedback shared by users
Matthieu Opitz successfully used Stampwell with Collagen-1, see this video
Queralt Tolosa, Centre for Genomic Regulation, Spain: "I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!“
Shai Eyal, UCSD, USA: "I used a custom Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!”
Matthieu Simion, TEFOR, France: "Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.“
Pour liquid agarose
Place the stamp
Reticulate the hydrogel
Remove the stamp
To image, just load your samples and image, sequence, transport at will.
To aggregate, seed your cells & let them aggregate.
V-shape & Embryo 1 specific protocol
Cross-dishes
Compatible with most 35 mm dishes and 6-well plates
Reusable
Use your stamp dozens of times
Safe for transport
Keep samples at the bottom of the wells, even when a dish is upside down
Long-term imaging over weeks
Samples lay on the bottom of the well. They can freely grow within it to image multiple times.
Alpha-numerical markers*
create an organized network of samples to identify the samples for a posteriori experiments
* for > 10 wells Stampwells
Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux
Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux
Diameter of the stamp: 26mm
7 shapes of wells to choose freely to fit your experiments:
V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
U Shape:
. Number of pins: 169
. Shape of the pins: U
Embryo 1 (aka Rectangular):
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm
Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm
Larvae 1:
. number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium
pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail
Larvae 2:
. Number of pins: 10. 5 pins for zebrafish larvae and 5 pins for the medaka larvae on the same stamp
. Shape of the pins: a prism.
. Length of the wells: 10 mm for the 5 long wells and 3 mm for the 5 small wells.
. Depth: 1 mm
. Width at the bottom of the wells: 0.3 mm
. Width of the upper side of the wells: 2 mm
Tell us your needs, and our product development team will begin defining the specifications for your custom Stampwell, perfectly suited to your samples and experimental requirements. They will produce it right after and it will be delivered to you in a few weeks.
Learn more about our Custom Program.
See examples below of custom stamps designed to maximize organoid generation in 6-well plates and create an organoid culture grid adapted to 12-well plates.
Get an automatic 10% discount for any combination of 2 Stampwells at once. Request your quote here.
The Stampwell most Frequently Asked Questions
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Camille Douillet
Esther Graudens
New projects at Idylle
Soon after that, researchers gave us their feedback of use and surprisingly, we discovered that they were using their stamps for culture and aggregation as well, in addition to imaging. Gaëlle had been thinking about it for a while actually. So she started designing a new model specifically for aggregation and Camille Douillet joined her to finetune it. On our side at Idylle, we relied on the design process and the production line we had previously built to launch that new stamp within 6 months, versus 18 months for the first models. The U-shape was born.
But the story does not end here! It started timidly, but then more and more researchers asked us to customize a stamp to image zebrafish and medaka embryos and larvae. So in addition to the Embyo 1, we decided to add 3 new models that would facilite imaging them up to 20 dpf.
The rest of the story remains to be written with you!"