Overview
Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids/organoids as well as fish embryos or larvae up to 20 dpf.
A technology originally designed by
Gaëlle Recher
for spheroids & organoids
Powered by the Mount(n) technology (UniverSlide) published in
Scientific Reports
Optimized by Idylle thanks to the Test Program feedback for zebrafish and medaka
Those little extras that make all the difference:
For stamps with more than 10 wells, alphanumeric markers make it possible to quickly identify 1 particular sample.
When you move your dish from the incubator to the microscope, the samples remain quietly in their wells (except the Stampwell made for aggregation)
Image your biological samples growing for several weeks in the same dish.

The 6 shapes
See the technical information about each shape in details.
Feedback from users
I successfully managed to use Stampwell with Collagen-1.
I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!
I used a Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!

Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.

Videos & images
Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux
Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux
Features

Cross-dishes
Compatible with most 35 mm dishes and 6-well plates

Reusable
Use your stamp dozens of times

Safe for transport
Keep samples at the bottom of the wells, even when a dish is upside down

Long-term imaging over weeks
Samples lay on the bottom of the well. They can freely grow within it to image multiple times.

Alpha-numerical markers*
create an organized network of samples to identify the samples for a posteriori experiments
* for > 10 wells Stampwells
Protocol
Global protocol

Pour liquid agarose

Place the stamp

Reticulate the hydrogel

Remove the stamp
To image, just load your samples and image, sequence, transport at will.
To aggregate, seed your cells & let them aggregate.
V-shape & Embryo 1 specific protocol
Technical information

Resources
- The most Frequently Asked Questions (FAQ)
- The V-shapes and Embryo 1 Datasheet
- The Embryo 2 Datasheet
- The Larvae 1 Datasheet
-
The Larvae 2
Datasheet
Kit description
-
Diameter of the stamp: 26mm
- 6 shapes of wells to choose freely to fit your experiments. See below.
-
Get an automatic 10% discount for any combination of 2 Stampwells at once.
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V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
Embryo 1 (aka Rectangular):
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm
Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm
Larvae 1:
. number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail
Larvae 2:
. Number of pins: 10. 5 pins for zebrafish larvae and 5 pins for the medaka larvae on the same stamp
. Shape of the pins: a prism.
. Length of the wells: 10 mm for the 5 long wells and 3 mm for the 5 small wells.
. Depth: 1 mm
. Width at the bottom of the wells: 0.3 mm
. Width of the upper side of the wells: 2 mm
Story

New projects at Idylle
But the story does not end up here! More and more researchers asked us to customize a stamp to image zebrafish and medaka embryos or larvae. So in addition to the Embyo 1, at Idylle we decided to add 3 new models that would facilite imaging them up to 20 dpf.
The rest of the story remains to be written with you!"
Combining Stampwell
You may also like to combine Stampwell
with other R&D products

Actiflash
Combine Actiflash with the V-shape
to immobilize and parallelize organoids for cell tracking,
or with the Larvae 2
to immobilize and parallelize embryos for in vivo cell tracking
Contact us about the stamps you're interested in!
Picture credit: Matthieu Simion - CNRS