Stampwell
The first stamp to mount 3D biological samples for parallelized microscopy experiments. Stampwell imprints arrays of wells in hydrogels and ensures the sample stillness, long-term medium-throughput and automatized multi-position image acquisition for a significant timesaving.
What is it for? Use it if you have tons of 3D samples to image. Or if you will spend hours to locate your sample in the dish. Or if you want to image over several weeks and observe how your biological samples grow. Or if your samples slip from one well to another when you move your dishes from the incubator to the microscope.
Formats, number of tips, manufacturing process... what else? Check all the technical info you need.
Designed by
Gaëlle Recher
Reviewed by Laetitia Andrique, Lydia Danglot, Sylvia Dyballa, Alessandro Furlan, Basile Gurchenkov, Jorge Torres-Paz
Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports.
Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux
Features
Medium/high throughput
42 objects can be loaded with one stamp
Each stamp can be used 50 times on average
Automatized multi-position image acquisition
Stampwell provides a constraint-free but stable and parallelized immobilization of the samples allowing automatized multi-position image acquisition
Long-term imaging
Because the sample is not embedded in the agarose pad but just laying on the bottom of a V-shaped well, it can freely grow within the well, enabling culture and image aquisition over days and weeks.
3 shapes to choose freely
- V-Shape 300µ: 7*6 pins in V-shape, forming a rectangle of 0.5 mm * 1 mm at the base and a circle of 300µm diameter on top
- V-Shape 500µ: 7*6 pins in V-shape, forming a rectangle of 0.5mm * 1mm at the base and a 500µm diameter circle on top
- Rectangular: 7*5 rectangular pins measuring 0.65 mm * 2 mm
Gallery
Cell-filled alginate capsule in the well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux
1 mn video protocol
Simplified protocol
hydrogel (agarose, alginate, phytagel, methylcellulose …)
35 mm petri dish, with or without glass bottom
a pipetman or pasteur pipette to load samples in wells
a cutter blade
a non-wounding spatula
and a stereomicroscope, ideally.
Manufacturing process: we produce countertyped silicone molds to manufacture polyurethane stamps.
External diameter: 26 mm. It is compatible with almost all 35-mm dishes.
Distance between the wells bottom and the dish bottom: 100 µm. Three feet have been designed so that Stampwell can lay on the glass of almost all glass bottom dishes, whatever their size.
Spotting: each well is identified with both a letter and a number. It allows the localization of the sample you want to further study.
Demolding: is made easy thanks to an ergonomic handle and curvature of the stamp’s base, as well as a notch allowing an object with a rounded tip to pass through to hold the hydrogel during demolding.
Lifetime: each stamp can be used 50 times (on average).
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Wells bottom diameter: 300 µm - Shape: circular
. Wells upper side: 1mm * 0,5 mm - Shape: rectangular
. Spacing between 2 wells: 1,70 mm on the lines and 2 mm on the columns
The right edge of the wells provides a slope to support and guide the pipette cone when loading the samples.
Lifetime: each stamp can be used 50 times (on average).
Number of pins: 42
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Wells bottom diameter: 500 µm - Shape: circular
. Wells upper side: 1mm * 0,5 mm - Shape: rectangular
. Spacing between 2 wells: 1,70 mm on the lines and 2 mm on the columns
The right edge of the wells provides a slope to support and guide the pipette cone when loading the samples.
Lifetime: each stamp can be used 50 times (on average).
Number of pins: 35
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Length * width: 2mm * 0,65 mm
. Spacing between 2 wells: 1,60 mm on the lines and 3 mm on the columns
Lifetime: each stamp can be used 50 times (on average).
Stampwell, Gaëlle Recher and us
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