Odoo • Image et Texte

Stampwell

The first stamp to mount 3D biological samples for parallelized microscopy experiments. Stampwell imprints arrays of wells in hydrogels and ensures the sample stillness, long-term medium-throughput and automatized multi-position image acquisition for a significant timesaving.


Designed by
 Gaëlle Recher
Reviewed by Laetitia Andrique, Lydia Danglot, Sylvia Dyballa, Alessandro Furlan, Basile Gurchenkov, Jorge Torres-Paz

Powered by the Mount(n) technology (UniverSlide) published in
Scientific Reports.

What is it for? Use it if you have tons of 3D samples to image. Or if you will spend hours to locate your sample in the dish.  Or if you want to image over several weeks and observe how your biological samples grow. Or if your samples slip from one well to another when you move your dishes from the incubator to the microscope.
How to use it? Read our simplified protocol or watch our 1mn video protocol
Formats, number of tips, manufacturing process... what else? Check all the technical info you need.
Why we chose it? Learn about the story of Stampwell and us
Prices from 225 €. See our offers.

Features

Medium/high throughput

42 objects can be loaded with one stamp

Each stamp can be used 50 times on average

Automatized multi-position image acquisition

Stampwell provides a constraint-free but stable and parallelized immobilization of the samples allowing automatized multi-position image acquisition

Long-term imaging

Because the sample is not embedded in the agarose pad but just laying on the bottom of a V-shaped well, it can freely grow within the well, enabling culture and image aquisition over days and weeks.

3 shapes to choose freely

- V-Shape 300µ: 7*6 pins in V-shape, forming a rectangle of 0.5 mm * 1 mm at the base and a circle of 300µm diameter on top
- V-Shape 500µ: 7*6 pins in V-shape, forming a rectangle of 0.5mm * 1mm at the base and a 500µm diameter circle on top
- Rectangular: 7*5 rectangular pins measuring 0.65 mm * 2 mm

1 mn video

 
 
Stampwell in 8 steps                                                                                                                                                                           

Gallery

 
 

Imprinted well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux

 
 

Cell-filled alginate capsule in the well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux

Simplified protocol








The material you will need is only:
hydrogel (agarose, alginate, phytagel, methylcellulose …), 
35 mm petri dish, with or without glass bottom,  
a pipetman or pasteur pipette to load samples in wells
a cutter blade
a non-wounding spatula
and a stereomicroscope, ideally.

Technical information

General information

Manufacturing process: we produce countertyped silicone molds to manufacture polyurethane stamps.
External diameter: 26 mm. It is compatible with almost all 35-mm dishes.
Distance between the wells bottom and the dish bottom: 100 µm. Three feet have been designed so that Stampwell can lay on the glass of almost all glass bottom dishes, whatever their size.
Spotting: each well is identified with both a letter and a number. It allows the localization of the sample you want to further study.
Demolding: is made easy thanks to an ergonomic handle and curvature of the stamp’s base, as well as a notch allowing an object with a rounded tip to pass through to hold the hydrogel during demolding.
Lifetime: each stamp can be used 50 times (on average).

V-shape 300 µm - For medium-size rounded 3D objects such as spheroids and organoids
Number of pins: 42
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Wells bottom diameter: 300 µm - Shape: circular
. Wells upper side: 1mm * 0,5 mm - Shape: rectangular
. Spacing between 2 wells: 1,70 mm on the lines and 2 mm on the columns
The right edge of the wells provides a slope to support and guide the pipette cone when loading the samples.
Lifetime: each stamp can be used 50 times (on average).

  

Number of pins: 42
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Wells bottom diameter: 500 µm - Shape: circular
. Wells upper side: 1mm * 0,5 mm - Shape: rectangular
. Spacing between 2 wells: 1,70 mm on the lines and 2 mm on the columns
The right edge of the wells provides a slope to support and guide the pipette cone when loading the samples.
Lifetime: each stamp can be used 50 times (on average).


 

Number of pins: 35
Tips imprint wells with the following dimensions:
. Depth of wells: 1mm
. Length * width: 2mm * 0,65 mm
. Spacing between 2 wells: 1,60 mm on the lines and 3 mm on the columns 
Lifetime: each stamp can be used 50 times (on average).



Stampwell, Gaëlle Recher and us

"The Mount(n) technology is the results of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into an hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies...). The design of the wells prevents the object from moving when the plate or dish is moved, does not apply any mechanical constrain to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary."

 Contact Gaëlle Recher about her technology

Odoo • Image and TextEsther Graudens
New projects at Idylle
"Few projects demonstrate with such acuity the virtues of a good reviewing! We were about to start the production of the stamps, when the reviewing showed that the stamp did not fit in all the dishes! Within a few hours, together with Gaëlle and our design office, we designed a new shape that fits everywhere. These back and forth discussions between the 3 of us were exciting and fruitful throughout the full design process. Together we have evolved the shape, the colour and the material so that Stampwell offers both an ergonomic grip and functionalities that meet the usage characteristics of all applications. We hope you will enjoy it!"
Contact us about your challenges to mount 3D samples