Stampwell
A family of stamps to democratise the 3D culture: from aggregating cells to mounting and imaging 3D samples.
The Stampwell stamps imprint arrays of wells in hydrogels.
Choose among the 3 shapes of wells (V-shape, U-shape or Rectangular shape) already available or imagine your own shape (My shape).
What is Stampwell intended for?
The U-shape Stampwell is meant to easily grow spheroids or organoids in a reproducible, fast, and cheap way.
The V-shape Stampwell is used to image them (2 different sizes).
And the Rectangular Stampwell is designed to image Zebrafish or Medaka embryos.
Whatever your sample, Stampwell is highly helpful if you have tons of 3D samples to image. It avoids spending hours to locate your sample in the dish. It prevents them from slipping from one well to another when you move your dish from the incubator to the microscope. And it allows to image and observe how your biological samples grow over several weeks.
Which Stampwell do you need? Check our technical info and make your choice.
Kit description
Designed by
Gaëlle Recher
Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports.
How to use the Stampwells
Stampwell News
See more feedback here.
New Shape Now Available: the U-Shape for aggregation
The stamp to easily grow spheroids or organoids in a reproducible, fast, and cheap way.
Stampwell U-shape has been co-designed with Camille Douillet.
The 3 shapes of wells already available
To grow spheroids and organoids
And / or image them, as well as Zebrafish & Medaka embryos.
Check all the custom shapes we also designed.
Community Feedback We Found Interesting
March 2021 - Matthieu Opitz successfully used Stampwell with Collagen-1
"I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!“
Queralt Tolosa, Centre for Genomic Regulation, Spain
"I used a custom Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!”
Shai Eyal, UCSD, USA
"Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.“
Matthieu Simion, TEFOR, France
Features
Cross-platform
Compatible with most 35 mm dishes and 6-well plates
Reusable
Use your stamp dozens of times
Medium culture conditioning
U-shape : the design of a groove increases the culture medium conditioning and cell aggregates survival
Long-term imaging over weeks
with V-shape and Rectangular : the sample just lays on the bottom of the well and can freely grow within it
Safe transport of 3D objects
V-shape and Rectangular keep your samples at the bottom of the wells, even when the dish is upside down
Automatized multi-position image acquisition
V-shape and Rectangular provide a constraint-free but stable and parallelized immobilization of the samples
Gallery
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Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux
Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux
1 min protocol for the V-shape & Rectangular Stampwell
Simplified protocol
A gallery of custom Stampwells we designed and tailored for specific usages. They are the result of what scientists engaged into our Test Program asked us to design for them.
The "6-well"
Maximizing organoid generation in 6-well plates
The "12-well"
Organoid culture grid adapted to 12-well plates
The "Oryzias"
Imaging medaka at various developmental stages
The "laid back"
Lateral positioning and imaging of zebrafish larvae
Our Custom Program allows you to tune one or more parameters of the Idylle tools to get your own design. Learn more.
Stampwell, Gaëlle Recher, her team and us
Esther Graudens New projects at Idylle
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Camille Douillet Co-designer of the U-shape Stampwell