Stampwell

A family of 6 stamps designed to make culture and imaging
of zebrafish embryos, medaka and organoids
as easy as cost-effective

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Overview

Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids/organoids as well as fish embryos or larvae up to 20 dpf.

A technology originally designed by Gaëlle Recher for spheroids & organoids
Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports
Optimized by Idylle thanks to the Test Program feedback for zebrafish and medaka

Those little extras that make all the difference:

For stamps with more than 10 wells, alphanumeric markers make it possible to quickly identify 1 particular sample.
When you move your dish from the incubator to the microscope, the samples remain quietly in their wells (except the Stampwell made for aggregation)
Image your biological samples growing for several weeks in the same dish.

The 6 shapes

See the technical information about each shape in details.

Feedback from users

I successfully managed to use Stampwell with Collagen-1.

Matthieu Opitz • Alveole, France

I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!

Queralt Tolosa • Centre for Genomic Regulation, Spain

I used a Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!

Shai Eyal • UCSD, USA

Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.

Matthieu Simion • TEFOR, France

Videos & images

Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux

Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux

Heart imaging in a Larvae 1 Stampwell
Top picture: green-heart 3dpf zebrafish larvae placed in ventral view. Middle & bottom pictures: 3dpf zebrafish larvae PFA-fixed, mounted in glycerol and inserted in Larvae 1 Stampwell (anterior to the left). Pictures were taken using a Leica Spectral Confocal SP5.
Credits: Dr. Giovanni Risato, Prof. Natascia Tiso and Alina Ramazanova - Department of Biology, University of Padova, Italy

Improved positioning of 2dpf zebrafish larvae using the Embryo 2 Stampwell
Credits: Dr Rui Monteiro - Institute of Cancer and Genomic Sciences, University of Birmingham, United Kingdom

Features

Cross-dishes

Compatible with most 35 mm dishes and 6-well plates

Reusable

Use your stamp dozens of times

Safe for transport

Keep samples at the bottom of the wells, even when a dish is upside down

Long-term imaging over weeks

Samples lay on the bottom of the well. They can freely grow within it to image multiple times.

Alpha-numerical markers*

create an organized network of samples to identify the samples for a posteriori experiments
* for > 10 wells Stampwells

Protocol

Global protocol

Pour liquid agarose

Place the stamp

Reticulate the hydrogel

Remove the stamp

To image, just load your samples and image, sequence, transport at will.

To aggregate, seed your cells & let them aggregate.

DOWNLOAD THE PROTOCOL

V-shape & Embryo 1 specific protocol

8 steps of the protocol of use, by Gaëlle Recher

Technical information

Resources

The most Frequently Asked Questions (FAQ)
The V-shapes and Embryo 1 Datasheet
The Embryo 2 Datasheet
The Larvae 1 Datasheet
The Larvae 2 Datasheet

Kit description

Diameter of the stamp: 26mm
6 shapes of wells to choose freely to fit your experiments. See below.
Get an automatic 10% discount for any combination of 2 Stampwells at once.

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V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular

V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular

Embryo 1 (aka Rectangular):
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm

Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm

Larvae 1:
. number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail

Larvae 2:
. Number of pins: 10. 5 pins for zebrafish larvae and 5 pins for the medaka larvae on the same stamp
. Shape of the pins: a prism.
. Length of the wells: 10 mm for the 5 long wells and 3 mm for the 5 small wells.
. Depth: 1 mm
. Width at the bottom of the wells: 0.3 mm
. Width of the upper side of the wells: 2 mm

Story

" The Stampwell family of products is born from a technology that I designed, called Mount(n). It is the result of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time I learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into a hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies...). The design of the wells pr events the object from moving when the plate or dish is moved, does not apply any mechanical constraints to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary. "

Gaëlle Recher

Esther Graudens
New projects at Idylle

" Together, we turned the Mount(n) technology into a family of research products. First, we have evolved the shape, the color and the material to offer an ergonomic grip and functionalities that meet the usage characteristics of most applications. Then, as we were about to start the production, the first testers found out that the stamp did not fit in all their dishes. Within a few days, together with Gaëlle and our design office, we redesigned a shape and in April 2021, the Test Program for the first Stampwell models, 2 V-shapes and Embryo 1, was released.
But the story does not end up here! More and more researchers asked us to customize a stamp to image zebrafish and medaka embryos or larvae. So in addition to the Embyo 1, at Idylle we decided to add 3 new models that would facilite imaging them up to 20 dpf.
The rest of the story remains to be written with you!"