Stampwell


A family of stamps to democratise the 3D culture: from aggregating cells to
 mounting and imaging 3D samples. 

Odoo • Image et Texte

The Stampwell stamps imprint arrays of wells in hydrogels.
Choose among the 3 shapes of wells (V-shape, U-shape or Rectangular shape) already available or imagine your own shape (My shape).

What is Stampwell intended for?
The U-shape Stampwell is meant to
easily grow spheroids or organoids in a reproducible, fast, and cheap way.
The V-shape Stampwell is used to image them (2 different sizes).

And the Rectangular Stampwell is designed to image Zebrafish or Medaka embryos.
Whatever your sample, Stampwell is highly helpful if you have tons of 3D samples to image. It avoids spending hours to locate your sample in the dish. It prevents them from slipping from one well to another when you move your dish from the incubator to the microscope. And it allows to image and observe how your biological samples grow over several weeks.

 Which Stampwell do you need? Check our technical info and make your choice.
 
Kit description 

Designed by  Gaëlle Recher
Powered by the Mount(n) technology (UniverSlide) published in Scientific Reports.

How to use the Stampwells


 
 

See more feedback here.

New Shape Now Available: the U-Shape for aggregation

The stamp to easily grow spheroids or organoids in a reproducible, fast, and cheap way.

Stampwell U-shape has been co-designed with Camille Douillet.

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Mock-up of the U-shape

The 3 shapes of wells already available

To grow spheroids and organoids
And / or image them, as well as Zebrafish & Medaka embryos.
Check all the custom shapes we also designed.

Odoo • Text and Image

Community Feedback We Found Interesting


March 2021 - Matthieu Opitz successfully used Stampwell with Collagen-1

"I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!
Queralt Tolosa, Centre for Genomic Regulation, Spain

"I used a custom Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!
Shai Eyal, UCSD, USA

Odoo • Text and Image
Odoo • Image and Text

"Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.“
Matthieu Simion, TEFOR, France

You want to test a Stampwell? Just register to its Test Program!

 GET IN THE TEST PROGRAM  

Features

  Cross-platform

 Compatible with most 35 mm dishes and 6-well plates

Reusable

Use your stamp dozens of times

Medium culture conditioning 

U-shape : the design of a groove increases the culture medium conditioning and cell aggregates  survival


Long-term imaging over weeks

with V-shape  and Rectangular  the sample just lays on the bottom of the well and can freely grow within it

   Safe transport of 3D objects

V-shape and Rectangular keep your samples at the bottom of the wells, even when the dish is upside down

Automatized multi-position image acquisition
V-shape and Rectangular provide a constraint-free but stable and parallelized immobilization of the samples


Customize your shape

My-Shape is your own design and number of wells 

Contact us for details

Gallery

 

Imprinted V-shape 300µ well in agarose
Soaked in fluorescein, negative rendering
Image credit: (c)Gaëlle Recher - Bordeaux

 
 

Cell-filled alginate capsule in the V-shape well, imaged in z
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
Image credit: (c)Gaëlle Recher - Bordeaux

1 min protocol for the V-shape & Rectangular Stampwell

 
 
The Stampwell V-shape and Rectangular in 8 steps                                                                                                                                                                           

Simplified protocol







The material you will need is only:
hydrogel (agarose, collagen, alginate, phytagel, methylcellulose …)
35 mm petri dish, with or without glass bottom 
a pipetman or pasteur pipette 
a cutter blade
a non-wounding spatula
and a stereomicroscope, ideally.

"My Shape"

A gallery of custom Stampwells we designed and tailored for specific usages. They are the result of what scientists engaged into our Test Program asked us to design for them.


The "6-well"

Maximizing organoid generation in 6-well plates


The "12-well"

Organoid culture grid adapted to 12-well plates


The "Oryzias"

Imaging medaka at various developmental stages

The "laid back"

Lateral positioning and imaging of zebrafish larvae

Our Custom Program allows you to tune one or more parameters of the Idylle tools to get your own design. Learn more.

Stampwell, Gaëlle Recher, her team and us

"The Stampwell family of products is born from a technology that I designed, called Mount(n). It is the result of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time I learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into a hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies...). The design of the wells prevents the object from moving when the plate or dish is moved, does not apply any mechanical constraints to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary."

Odoo • Image and TextEsther Graudens
New projects at Idylle
"Together, we turned the Mount(n) technology into a family of research products. First, we have evolved the shape, the color and the material to offer an ergonomic grip and functionalities that meet the usage characteristics of most applications. Then, as we were about to start the production, the first testers found that the stamp did not fit in all their dishes. Within a few days, together with Gaëlle and our design office, we designed a new shape. And in April 2021, Stampwell for imaging was released with 3 different shapes: 2 V-shapes and the Rectangular one. Soon after that, researchers gave us feedback on the fact that they used their stamp for culture and aggregation as well. Gaëlle had been thinking about it for a while so she started designing it. Camille Douillet joined her to finetune it. At Idylle, we relied on the design process and the production line we had previously built to launch that new stamp dedicated to the aggregation. We managed to release it in 6 months, versus 18 months for the first Stampwells. We hope you will enjoy this new stamp as much as the first ones!"

Odoo • Image and TextCamille Douillet 
Co-designer of the U-shape Stampwell
Contact us about your challenges to mount 3D samples