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Stencell

 

 

Designed by Vincent Studer, Pierre-Olivier Strale and Aurélien Pasturel 

"While developing an innovative micropatterning technology on glass coverslips, we had to optimize our protocole and thus screen a lot of conditions with expensive reagents. We could have used glass-bottom 96wp but we found them expensive and inconvenient for reproducible surface treatment. We thus started to develop our own low-volume fluid handling method. We quickly discovered that hydrophobic perforated PDMS films could act as reversible boundaries for liquids. With simple craft cutting robot, we were able to fabricate on-demand stencils of any shape and size. By filling those stencils with microliters droplets, we end up with a very versatile and reproducible high-throughput fluid handling solution."  

Contact Vincent, Pierre-Olivier and Aurélien
about their technology




    STENCELL & EXAMPLES OF RESULTS

    APPLICATIONS

     TECHNOLOGY FEATURES

     HOW TO USE STENCELL

    USERS FEEDBACKS

     PUBLICATIONS

  STENCELL & EXAMPLES OF RESULTS

Stencell is a ready-to-use sticky foil with compartments that you can use for cellular confinement. It is designed to standardize the experiments and to minimize the consumption of reagents.

Stencell with colored solutions
Image credit: © Pierre-Olivier Strale

 
 
  COS-7 cells colonizing a gap induced by the removal of a PDMS stencil.
Images shot with a 20X objective during 18 hours (1 image every 2 min) with an inverted microscope.

Image credit:
© Pierre-Olivier Strale
Odoo • Image et Texte
Confined COS-7 cells stained for actin and nucleus. 
Image credit:  © Pierre-Olivier Strale
Odoo • Image et Texte
  Confined COS-7 cells stained for actin and nucleus.
Image credit:
© Pierre-Olivier Strale

  APPLICATIONS

  TECHNOLOGY FEATURES

Standardized, reproducible environment: Wound healing experiments are traditionaly done by scratching a confluent monolayer, leading to poor reproducibility and non-physiological starting conditions. Stencell guarantees a collective cell migration in standardized, reproducible environments.

Removable multiwell stickers: Stencells are thin sheets of silicone that stick tightly to glass and most plastics with no need for glue, meaning they can be removed later on. This, combined with their multiwell format, can create removable boundaries between cell populations or allow you to simply run more experiments per sample.

   HOW TO USE STENCELL

 
 

  PUBLICATIONS