Stencell

PDMS gets out of the clean room!
The no-brainer solution for all your micro-volume experiments, anywhere.

Odoo • Image et Texte


What is it intended for? Use it notably for wound-healing assay & immunocytochemistry experiments. It is very helpful when you are working with super expensive reagents or very rare cell lines, and when you want to test various experimental hypothesis.
 No limit! Choose a ready-to-use design (Solo - Quartet - Nonet - Presto - Allegro). Or design your own shapes & stack them freely.
 The "Starter kit" provides 2 sheets of 50 Stencells each. Kit descriptions, designs, Technical datasheet and FAQ

D esigned by Vincent Studer, Pierre-Olivier Strale and Aurélien Pasturel 
They were hosted by the Cell Organ-izers joint research laboratory (CNRS-Alvéole).
Powered by the Stencil technology published in Advanced Healthcare Materials .

Odoo • Text and Image

Stencell news

 
 

Stencell for wound-healing experiments

 
 
COS-7 cells colonizing a gap induced by the removal of a PDMS stencil.
Images shot with a 20X objective during 18 hours (1 image every 2 min) with an inverted microscope.

Image credit:
© Pierre-Olivier Strale
Odoo - Sample 1 for three columns
 Odoo - Sample 2 for three columns

  Stacking the 5 designs of Stencell

Odoo • Image and Text

Features


Design your PDMS


Our design process offers total freedom to turn your idea into a PDMS. 

We produce it with the highest standards of quality, whether you want 1 or 100 copies


Stack and play


Compose flow channels and chambers, stacking different designs of Stencell


Stick it to a variety of culture labware

So far, it has been successfully stuck on: 

  • Glass slides

  • Plastic dishes

  • Transwell(R) inserts

  • Polyacrylamide gels


Remove it

Stencell is not glued. It can then be removed to:

  • Trigger cell migration

  • Switch from flow chamber to open window

Very useful for wound healing experiments


It is compatible with imaging
 

These thin sheets of silicone are fully transparent, without autofluorescence


Parallelized and standardized experiments

One multiwell Stencell can perform several experimental conditions


It saves samples and reagents
 

A few microliters only are required

  Gallery of results

  Simplified protocol

  Product Information

The "Starter kit"
- 2 sheets of 50 Stencells each
- Either the same design for the 100 Stencells, or 2 different designs (one design per sheet).

The "Get your own design" kit:
- 1 design study to develop your project
- 2 sheets of 50 Stencells each (priced as the ones available in the "Starter kit")
- You can mix 1 sheet of your design with 1 sheet of the already existing ones.

The designs:
Each sheet is composed of 50 identical Stencells.
Upon receipt of your purchase order, you will be able to choose either the same design for your 2 sheets, or 2 different designs for your 2 sheets (1 per sheet of 50).

- Solo: 1 circular well - Diam. 12 mm
- Quartet: 4 circular wells - Diam. 3 mm
- Nonet: 9 circular wells - Diam. 3 mm
- Presto: 2 oblong wells spaced by 0.35 mm
- Allegro: 2 oblong wells spaced by 0.62 mm


Stencell and us

"While developing an innovative micropatterning technology on glass coverslips, we had to optimize our protocole and thus screen a lot of conditions with expensive reagents. We could have used glass-bottom 96wp but we found them expensive and inconvenient for reproducible surface treatment. We thus started to develop our own low-volume fluid handling method. We quickly discovered that hydrophobic perforated PDMS films could act as reversible boundaries for liquids. With simple craft cutting robot, we were able to fabricate on-demand stencils of any shape and size. By filling those stencils with microliters droplets, we end up with a very versatile and reproducible high-throughput fluid handling solution."


Contact the team about their technology.
Odoo • Image and Text Esther Graudens
New projects at Idylle

"Sometimes research needs a high level of versatility. This is exactly what appealed me when I met Vincent, Pierre-Olivier and Aurelien. They were hosted by the Cell Organ-izers joint research laboratory, set up by CNRS and our sister company Alveole. So they naturally turned to us when they decided to look for a partner to release their stencils. We knew that Stencell would be so useful to a lot of researchers who could save reagents and cells while testing new ideas and experimental conditions on small volumes. So we said yes immediately. On top of that, the three of them have proven to be highly reactive and proactive, engaged and enthusiastic. They love as much as we do testing new ideas and suggest others. It was a great pleasure to go through our industrial process together!"

Contact us if you want to discuss your cellular confinement projects . Or just share insights on a research project.