Virusly

Bio-Nanoparticles that look like viruses but are not viruses!
Prepared with HIV virus, they allow you to calibrate or check your microscope easily and safely out of the BSL3.

 Features      Protocol      Story
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What is it for?  Use it if you want to calibrate with Bio-Nanoparticles your NTA*, DLS*, ILM* (Videodrop) OR your fluorescence microscope OR if you want to mimic viruses avoiding the BSL3 lab.  

Designed by  Delphine Muriaux
Powered by the VLP technology published in Scientific Reports

* NTA: Nanoparticules Tracking Analysis - DLS: Dynamic Light Scattering - ILM: Interferometry Light Microscopy -  VLP: virus-like particles

Features

Monodispersed Bio-Nanoparticles

150 +/- 10% nm diameter

 


Fluorescent tag

Virusly can be provided under non-fluorescent or fluorescent form: mCherry, eGFP or both or mEos2 

Stable for 6 months

Virusly can be stored 6 months in a fridge 

Safe

Virusly are non-infectious virus-like nanoparticles

 
 
Virusly observed with the Videodrop (courtesy of Myriade and CEMIPAI)                                                                                                                        

Gallery

Simplified protocol


Note: if you want to know more about the Videodrop from Myriade, click here.


The material you will need is only:
> Hardware:   
Fluorescence microscope or ILM or DLS or NTA
Humidified Incubator @ 37°C and 5% CO2 in case of fluorescence microscopy

> Consumables:
Appropriate coverslip (e.g. 25mm for AttoFluor Cell Chamber)
Eppendorf tube (0,5 or 1.5mL)
Appropriate coverslip holder: e.g. Ludin Chamber OR AttoFluor Cell Chamber...

> Reagents:
Virusly sample
Filtered TNE buffer pH 7,4 OR your favorite filtered buffer for dilution (PBS) OR cell culture media ...

Virusly, Delphine Muriaux and us

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 Contact Delphine Muriaux about her technology

Odoo • Image and TextEsther Graudens
New projects at Idylle
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Getting a hard time with your viral proteins or fluorescent tags?