AgarSqueezer is a microscope slide chamber equipped with a molded agar-based compression system. This innovation helps analyzing cell response to long-term mechanical confinement within a physiological rigidity range.
What is it intended for? Use if you want to analyze how your cells will react if we squeeze them for a prolonged period. Use it if you want to study how mechanical confinement affects drug cell resistance. Use if you want to perform immunostaining in situ.
Designed by Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière
Lab on a chip
The Test Program is open!
Registration is starting on: September 21, 2022
The campaign is closing on: December 20, 2022
The objectives of this 1st campaign are to test:
Cell lines that have not been tested by the team of developers (they already tested hematopoietic TF1 cells, Parental TF1-GFP and leukemic BCR-ABL transformed TF1-BA cells, ML2 leukemic cells, adherent HS27A cells)
Bilayers of cell cultures
Spheroids or organoids
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the AgarSqueezer stage adapter to mount up to 2 AgarSqueezers on the microscope stage
A 16G or 20G flat-cut needle
To make holes in the agar gel, to allow the addition of culture medium or drug during the experiment
What you need
to easily perform your experiments
And a wafer
to mold pillars in the agar gel
We provide 3 heighs:
. 2.5 µM to highly confine cells
. 5 µM to moderately confine cells
. 30 µm not to confine cells (negative control)
Tunable stiffness in a physiological range [1-150] kPa
Use of agarose as a cheap and biocompatible polymer
Permeability to small molecules &
Open access to the reservoir
Easy medium renewal
Possibility to add drugs, and reagents for immunostaining in-situ
Long-term analysis of the cell adaptation to confinement
Up to several days, for time-lapse studies
Compatibility with multiple microscopy techniques
Confocal, spinning, super-resolution)
Open access for microscope objectives
Use of optical glass coverslip to make cells grow
Easy to recover coverslip with cells for subsequent molecular analysis
FACS, qPCR, Western-Blot, Immunofluorescence (possible in situ)
Easy to assemble and disassemble the system
Autoclavable & reusable systems
Study of the specific impact of mechanical forces on the biology of cells
Gas permeability of the system allows to get rid of the hypoxia conditions
Includes 1 AgarSqueezer device + 1 adaptor to the microscope platine + 1 * 16G flat cut needle + 1 * 20G flat cut needle
Includes 2 AgarSqueezer devices + 1 adaptor to the microscope platine + 1 * 16G flat cut needle + 1 * 20G flat cut needle
Includes 1 wafer to mold agarose. Height to choose among 2.5, 5, 30 µ.
Includes 2 wafers to mold agarose. Height to choose among 2.5, 5, 30 µ.
AgarSqueezer's simplified protocol
AgarSqueezer and us
On their side, Veronique's team was looking for a way to analyze the effect of long-term confinement to perform time-lapse analysis, up to several days, with the ability to add a drug at any time.
We all wanted to find a device that would also meet the requirements of subsequent classical molecular analysis (easy cell culturing and cells recovery, qPCR, western-blot, in situ immunostaining) as well as biophysical image-based analysis (high-resolution microscopy and video-microscopy). But we could not find the perfect device for that.
So all together, we decided to collaborate to have back-and-forth feedback on the perfect set-up. We rapidly identified agarose as an interesting material to get medium and oxygen renewal, with no drug adsorption. We built several prototypes, improving precise control of the confinement, avoiding destruction of the gels and leakage of the culture medium! And then we came up with the Softconfiner device, that we published and decided to transfer.
Today, we believe that the AgarSqueezer could be of interest for many researchers willing to better understand how mechanics can regulate cell behavior. We hope that researchers from different communities will also find it a useful tool!"
Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière
New projects at Idylle
If you want to know more about the journey that we followed together to turn Softconfiner into AgarSqueezer, then have a look at the AgarSqueezer story of tech transfer . "