BrightER
A transfection-free solution to image the full endoplasmic reticulum within minutes
BrightER is a selective, cell-permeable endoplasmic reticulum probe conjugated to a bright photostable rhodamine dye. Just dilute and drip 10µL per mL of cell culture medium and you immediately visualize the endoplasmic reticulum (ER) structure up to the nucleus in live cell experiments. So far, all experiments ended up with full ER structure images. See the Test Program if you want to test it on your cells.
A technology designed by Raphaël Gaudin and Yonis Bare.
A tech transfer story that started with a serendipitous discovery. Learn about this journey in the Story section.
Transfection-free
Stain your most recalcitrant cells and preserve your cell physiology
Non-toxic
Image as long as you want, keep growing your cells, and repeat. No need to wash out!
ER specific
Staining of the entire ER in continuity with the nuclear membrane
Fast
No need for complicated resuspension. Simply add BrightER to your culture medium and image within minutes
Universal
Compatible with both human and non-human cell types
Beautiful
Get a bright and homogeneous signal, suitable for low laser intensity imaging
Read the Safety Datasheet.
Colour: Red
Wavelength range: Ex:557/Em:576.
Dye type: Rhodamine
For use with: Fluorescence microscopes, Confocal microscopes
Form: Liquid
SubCellular localization: Endoplasmic reticulum
Lifetime: up to 4 months at -20°C
Applications: live imaging of ER morphological features and dynamic processes such as membrane contact sites, intracellular trafficking, secretory pathways, viral infections, translation, protein folding and unfolded protein response (UPR), nuclear envelop formation upon mitosis, cellular calcium regulation and many others.
Tested and validated on (cell types): HeLa, U-2 OS (human osteosarcoma), SVG-A (human astrocytes), Vero E6 (monkey kidney epithelial cells)
Suggestions for use: Diluted BrightER solution may be added directly in full media. In most cases, a final 1:100 dilution is sufficient for immediate and bright ER staining; however, optimization may be needed for some cell types, conditions, and applications. BrightER is detected through standard TRITC and DsRed filters.
1 kit includes:
- 1 vial containing 10µL of concentrated BrightER solution in DMSO
- 1 vial containing 90µL of PEG-400 dilution solution
Number of experiments with 1 kit: 20
Seed
your cells
Add BrightER
directly to your full culture medium
Image
Raphaël Gaudin and Yonis Bare
“Our team has a long-standing interest in studying the spatiotemporal dynamics of viral infections. To this end, we develop tools to characterize how viruses penetrate into cells and reach their replication site, and engineer antiviral strategies to inhibit those steps. One day, we serendipitously noticed that one of the fluorescent molecules we had synthesized was unable to impact viral infection, but specifically localized on the endoplasmic reticulum. We decided to explore this further. Experiments indicated that this molecule was indeed a very good ER marker. It did not show apparent side effect on the cells: good cell viability, “normal” ER shape, no impact on secretory function.
When we met Aurelien Pasturel from Idylle, we realized that we could share it with the community of biologists interested in ER morphologies and functions. We were really tempted by this new adventure, and here we are!"
"The first time I went to Montpellier to talk to an audience of innovative researchers about our technology transfer solution, Raphaël took the opportunity to arrange a meeting. He came to see me the day following the presentation with a whole bunch of projects under his belt. Our exchanges led us to his surprising discovery of an excellent fluorescent probe specific of the endoplasmic reticulum while working on a marked anti-viral. A beautiful story of serendipity that marked the beginning of our collaboration.".
Aurelien Pasturel
New projects, Idylle