Chitozen

The 1st functionalized microscope coverslip to image live bacterial cells & study their growth and behavior. 

Odoo • Image et Texte


What is it for?  
Use it if you want to image E. coli bacteria both still and alive under the microscope. Or if you want to perform long-term imaging of bacteria. Or if you want to change the growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the E. coli bacteria new comportment under the microscope.

Designed by  Tâm Mignot, Olivier Theodoly, Amandine Desorme, Guillaume Sudre and Laurent David
Published in  mBio

 See below 2 videos with E. coli.

 
  
 
 

E. coli monolayers on Chitosan
© 2019 Tréguier et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Visualization of Pal mCherry at septum in E. coli (W3110 Pal mCherry), by 3D SIM microscopy, in M9 medium and using Chitozen.
Credit : Amandine Desorme - LCB - CNRS - 2021

Features

Long lasting


A bench-stable surface coated with chitosan, the most efficient way to immobilize your E. coli bacteria on a microscope coverslip

6 independent channels


Either perform up to 6 experiments at the same time or use 1 channel one day, and the others later

Full compatibility with most of your conditions of experiments

 Optical microscopy techniques: TIRF, super-resolution (3D-SIM)

 Size: the coverslip dimension (25x75 mm) is compatible with the most common available sticky slides and microscope stages.

  Compatible with advanced microfluidic techniques, nanolithography, PDMS 

Fast


Use it the same day it is prepared

Gallery

Simplified protocol

 
 




The material you will need:
Hardware

µ-Slide Microscopy Rack
Eppendorf® Centrifuge 5430 & Rotor 5430R A-2-MTP
Clamp and adapter for sticky slides 

Consumables

Bottomless 6 channels sticky slides (sterilized and welded in a gas-permeable packaging)

Reagents

LB medium
LB ½ medium 
Milli-Q® water

Cells

Escherichia coli.

Go further: perfuse the system with flow

Odoo • A picture with a caption
Chitozen coverslips allow bacterial cells to be immobilized even under a flow.

Chitozen and us

"We wanted to understand how our bacteria, Myxococcus xanthus, moves on a surface. So we tried to design a smart microscopy system to observe it. But there was a problem: the Myxococcus cells were not adhering to our glass slides. When we read a paper on how people use chitosan to get their bacteria better move on plates, we got an idea: maybe we could coat our glass slides with some chitosan? We tested it. And it worked! We then implemented our prototype with a microfluidics system to test the real time response of our bacteria to antibiotics. Now we’re thrilled to share it with the community of research! We hope that people working on bacteria will appreciate to use Chitozen because it is a very easy system to run that combines microfluidics and high-end microscopy. We are looking forward to getting feedback of use: it will mean that we have developed a device useful to the community and that is always a deep satisfaction!"
Tâm Mignot, Olivier Théodoly, Amandine Desorme, Guillaume Sudre, Laurent David

 Contact the developers about their technology
Odoo • Image and Text

Odoo • Image and TextEsther Graudens
New projects at Idylle
" We really loved the way all the team, whether in Marseille or in Lyon, was deeply concerned by the tiniest detail and willing to prepare the best ever product for the community. So if you want to know more about how we co-designed and produced Chitozen starting with chitosan, then catch an eye on the story of Chitozen.
And if you are interested in seeing what you can do with chitosan-coated coverslips, you can have a look at this study published by the team of developers in 2019. They've managed to use chitosan-coated coverslips to promote the growth of cells without any deleterious effect on their physiology, allowing them to measure the antibiotic susceptibiliy of a diversity of clinical strains with an excellent accuracy in a very short period of time. Read all about it here: mBio, 2019"

Contact us about your challenges to image your bacteria