Chitozen
The 1st functionalized microscope coverslip to image live bacterial cells & study their growth and behavior.
What is it for?
Use it if you want to image E. coli bacteria both still and alive under the microscope. Or if you want to perform long-term imaging of bacteria. Or if you want to change the growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the E. coli bacteria new comportment under the microscope.
Designed by
Tâm Mignot, Olivier Theodoly, Amandine Desorme, Guillaume Sudre
and
Laurent David
Published in
mBio
See below 2 videos with E. coli.
Features
Long lasting
A bench-stable surface coated with chitosan, the most efficient way to immobilize your E. coli bacteria on a microscope coverslip
6 independent channels
Either perform up to 6 experiments at the same time or use 1 channel one day, and the others later
Full compatibility with most of your conditions of experiments
Optical microscopy techniques: TIRF, super-resolution (3D-SIM)
Size: the coverslip dimension (25x75 mm) is compatible with the most common available sticky slides and microscope stages.
Compatible with advanced microfluidic techniques, nanolithography, PDMS
Fast
Use it the same day it is prepared
Gallery
Simplified protocol
Hardware
µ-Slide Microscopy Rack
Eppendorf® Centrifuge 5430 & Rotor 5430R A-2-MTP
Clamp and adapter for sticky slides
Bottomless 6 channels sticky slides (sterilized and welded in a gas-permeable packaging)
LB medium
LB ½ medium
Milli-Q® water
Escherichia coli.
Go further: perfuse the system with flow
Chitozen and us
Tâm Mignot, Olivier Théodoly, Amandine Desorme, Guillaume Sudre, Laurent David
Contact the developers about their technology
Esther Graudens New projects at Idylle
And if you are interested in seeing what you can do with chitosan-coated coverslips, you can have a look at this study published by the team of developers in 2019. They've managed to use chitosan-coated coverslips to promote the growth of cells without any deleterious effect on their physiology, allowing them to measure the antibiotic susceptibiliy of a diversity of clinical strains with an excellent accuracy in a very short period of time. Read all about it here: mBio, 2019"