BiographyBiologist by training, I came to the Molecular Electrochemistry Laboratory in 1999 to bring my skills in biochemistry and molecular biology. I started by working on a fundamental research theme aimed at understanding the functioning of redox membrane co-factors. From 2003, together with my colleague Benoit Limoges, I developed a theme focusing on analytical electrochemistry. The aim was to use electrochemistry as a method of ultra-sensitive biological target detection. We initially worked on the concepts of electro-enzymatic biosensors offering cost advantages and robustness compared to optics. Then, starting in 2005, we sought to apply these same advantages to real-time PCR approaches, leading to Leox technology.
Research TopicsTogether with my team, we are working on the development and study of different supramolecular assemblies by electrochemical methods, whether in homogeneous solution or immobilized at the electrode surface. The design, synthesis and assembly of molecules with multiple chemical and physico-chemical properties and, depending on the purpose, the functionalization of electrodes by immobilizing these molecules allows the development of electrochemical devices for analytical applications and fundamental studies.
Fields of interest
Helixohm probes are extremely stable electroactive compounds used as molecular probes for the detection of nucleic acids in molecular biology. They consist in a range of probes with different standard electroactive potentials.
Several PublicationsMoreau M, Delile S, Sharma A, Fave C, Perrier A, Limoges B, Marchal D. Detection of a few DNA copies by real-time electrochemical polymerase chain reaction. Analyst. 2017 Sep 8;142(18):3432-3440. https://pubs.rsc.org/en/content/articlelanding/2017/AN/C7AN00978J
Martin A, Grant K, Stressmann F, Ghigo JM, Marchal D, Limoges B. Ultimate single-copy DNA detection using real-time electrochemical LAMP. ACS sensors. 2016, 1 (7), 904-912. https://pubs.acs.org/doi/abs/10.1021/acssensors.6b00125
Deféver T, Druet M, Evrard D, Marchal D, Limoges B. Real-time electrochemical PCR with a DNA intercalating redox probe. Anal Chem. 2011 Mar 1;83(5):1815-21. https://pubs.acs.org/doi/10.1021/ac1033374
Kivlehan F, Mavré F, Talini L, Limoges B, Marchal D. Real-time electrochemical monitoring of isothermal helicase-dependent amplification of nucleic acids. Analyst. 2011 Sep 21;136(18):3635-42. https://pubs.rsc.org/en/content/articlelanding/2011/AN/c1an15289k
Limoges B, Marchal D, Mavré F, Savéant JM, Schöllhorn B. Theory and practice of enzyme bioaffinity electrodes. Direct electrochemical product detection. J Am Chem Soc. 2008 Jun 11;130(23):7259-75. https://pubs.acs.org/doi/abs/10.1021/ja7102845
Andrieux CP, Limoges B, Marchal D, Savéant JM. Redox enzymes immobilized on electrodes with solution cosubstrates. General procedure for simulation of time-resolved catalytic responses. Anal Chem. 2006 May 1;78(9):3138-43. https://pubs.acs.org/doi/10.1021/ac052176v
Limoges B, Marchal D, Mavré F, Savéant JM. High amplification rates from the association of two enzymes confined within a nanometric layer immobilized on an electrode: modeling and illustrating example. J Am Chem Soc. 2006 May 10;128(18):6014-5. https://pubs.acs.org/doi/10.1021/ja060801n
Marchal D, Pantigny J, Laval JM, Moiroux J, Bourdillon C. Rate constants in two dimensions of electron transfer between pyruvate oxidase, a membrane enzyme, and ubiquinone (coenzyme Q8), its water-insoluble electron carrier. Biochemistry. 2001 Feb 6;40(5):1248-56. https://pubs.acs.org/doi/10.1021/bi002325y