« I wish I could be as bright as the probes I develop »
BiographyI started studying biology and chemistry at the Pierre and Marie Curie University (Paris VI), where I finally graduated in molecular chemistry. I started my PhD at the Ecole Normale Supérieure (ENS) in Paris in 2004 on the medicinal chemistry of carbohydrates, especially in the synthesis of oligosaccharides. In 2008, I joined Prof. Seeberger's group as a postdoctoral fellow (ETH Zurich and Max Planck Institute Berlin) where I worked on the automated synthesis of Glycocsaminoglycans and on multivalent carbohydrate systems. In 2010, I returned to Paris where I changed the subject of research and started to develop fluorescent probes and nanostructured sensors. In 2013, I was recruited at the National Center for Scientific Research (CNRS) where I currently work at UMR-7021 (Strasbourg) and where I develop fluorescent and fluorogenic tools for bio-imaging.
Research TopicsMy research focuses on the development of molecular and nanostructured fluorescent probes for bio-imaging. Our probes are based on different systems providing effective fluorescent ignition effects or spectral modifications when detecting cellular constituents (plasma membrane, lipid droplets) or cellular events (calcium detection, pH variation, detection of reducing / oxidizing environment). Our goal is to develop brightt, versatile fluorophores that can be used in both multiphoton and super-resolution microscopy. I am also working on fluorescent and very bright nanoemulsions and small polymer nanoparticles that can be easily functionalized to develop fluorescent nanobiosensors.
Fields of interest
Development of fluorescent tools; Fluorogenic probes; Fluorescent nanoparticles; Bioimaging
Organelle-specific probes; Calcium probes; PH probes
Plasma membrane; mitochondria; Lipid droplets
Multiphoton imaging; Super-resolution imaging Confocal microscopy
MemBright has been designed to ensure efficient, fast, homogeneous and bright fluorescence staining of cell plasma membranes. MemBright is compatible with various samples, including 2D and 3D cell cultures, organoids and tissues.
(1) Collot, M.; Ashokkumar, P.; Anton, H.; Boutant, E.; Faklaris, O.; Galli, T.; Mély, Y.; Danglot, L.; Klymchenko, A.S. MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience, Cell Chem. Biol. 2019, doi: 10.1016/j.chembiol.2019.01.009. Author copy open access: www.biorxiv.org/content/10.1101/380451v1
(2) Collot, M.; Boutant, E.; Lehmann, M.; Klymchenko, A. S. BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe. Bioconjug. Chem. 2018. https://doi.org/10.1021/acs.bioconjchem.8b00828.
(3) Collot, M.; Fam, T. K.; Ashokkumar, P.; Faklaris, O.; Galli, T.; Danglot, L.; Klymchenko, A. S. Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues. J. Am. Chem. Soc. 2018, 140 (16), 5401–5411. https://doi.org/10.1021/jacs.7b12817.
(4) Collot, M.; Bou, S.; Fam, T. K.; Richert, L.; Mély, Y.; Danglot, L.; Klymchenko, A. S. Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push–Pull Fluorophore. Anal. Chem. 2018. https://doi.org/10.1021/acs.analchem.8b04218.
(5) Despras, G.; Zamaleeva, A. I.; Dardevet, L.; Tisseyre, C.; Magalhaes, J. G.; Garner, C.; Waard, M. D.; Amigorena, S.; Feltz, A.; Mallet, J.-M.; et al. H-Rubies, a New Family of Red Emitting Fluorescent PH Sensors for Living Cells. Chem. Sci. 2015, 6 (10), 5928–5937. https://doi.org/10.1039/C5SC01113B.
(6) Ponsot, F.; SHEN, W.; Ashokkumar, P.; Audinat, E.; Klymchenko, A. S.; Collot, M. PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience. ACS Sens. 2017. https://doi.org/10.1021/acssensors.7b00665.
(7) Collot, M.; Ponsot, F.; Klymchenko, A. S. Ca-NIR: A Ratiometric near-Infrared Calcium Probe Based on a Dihydroxanthene-Hemicyanine Fluorophore. Chem. Commun. 2017, 53 (45), 6117–6120. https://doi.org/10.1039/C7CC02418E.
(8) Collot, M.; Wilms, C. D.; Bentkhayet, A.; Marcaggi, P.; Couchman, K.; Charpak, S.; Dieudonné, S.; Häusser, M.; Feltz, A.; Mallet, J.-M. CaRuby-Nano: A Novel High Affinity Calcium Probe for Dual Color Imaging. eLife 2015, 4, e05808. https://doi.org/10.7554/eLife.05808.
(9) Collot, M.; Loukou, C.; Yakovlev, A. V.; Wilms, C. D.; Li, D.; Evrard, A.; Zamaleeva, A.; Bourdieu, L.; Leger, J.-F.; Ropert, N.; et al. Calcium Rubies: A Family of Red-Emitting Functionalizable Indicators Suitable for Two-Photon Ca2+ Imaging. J. Am. Chem. Soc. 2012, 134 (36), 14923–14931. https://doi.org/10.1021/ja304018d.