Karine Monier picture

  "Long-term sparkling is all to gather motivation."  

  Karine MONIER

       Institut Neuromyogène, Lyon


               

Who is Karine Monier ?

"I was born in Algiers in 1969. I followed a biological cursus at Grenoble University and then chose a lab mixing computing sciences and microscopy imaging for my Ph.D with Michel Robert-Nicoud on genome mapping by fluorescence in situ hybridization obtained in 1997. Attracted by live-cell imaging, I decided to join the Kevin Sullivan lab at TSRI in San Diego to work on centromeres and kinetochores. In 2004, I was selected to start my junior group at ENS Lyon on Epigenetics of pericentromeres. In 2007, I moved to a recent onsite transdisciplinary lab to work with chemists and physicists, where I was recruited as research engineer at CNRS in 2009. I started to work with Philippe Bouvet's  group on nucleolin and jointly developed a new project on super-resolution microscopy with a transdisciplinary consortium in the Joliot Curie Lab."

Dedication to ensure up to 3,5 months, stable blinking for dSTOM microscopy 

Researchers using dSTORM imaging were constantly constrained by the short lifespan and inconsistency of standard imaging buffers. The fluorescence signals would fade too quickly, limiting the potential for long-term observation and intricate analysis.

Karine and her team, driven by his dedication to practical innovation, took on this challenge with a simple yet ambitious question: What if there was a buffer that could last up to several months after mounting, increases blinking events, stay stable, and significantly simplifies researchers workflow by limiting interruptions due to buffer renewals?

After countless experiments and refinements,  the team developed EverSpark, a long-lasting buffer that revolutionizes dSTORM imaging. Designed to ensure stability over extended sessions, EverSpark provides researchers with the freedom to focus on their observations, confident in the reliability of their results.

EverSpark V2 – Pushing beyond limits with  green, yellow & far-red fluorophores

With the second version of EverSpark, the improvements are game-changing. In addition to yellow and red/far-red fluorophores, this version is also compatible with green channel fluorescence signals,  unlocking the potential for 3-colour dSTORM imaging. Blinking stability has been extended from two months to an incredible 3.5 months. The new formulation enhances blinking events, capturing even more crucial molecular details with greater precision. EverSpark V2 is not just an upgrade, it’s a testament to the power of continuous innovation in scientific research.


                          Arnaud Favier, Karine Monier and Christophe Place

 No more rushing when imaging 

EverSpark is a solution that transforms workflows in fluorescence microscopy. With its ability to maintain fluorescence signals up to 3.5 months. This solution allows researchers to push the boundaries of super-resolution imaging. From observing dynamic molecular interactions to mapping cellular structures in exquisite detail, EverSpark has become an essential tool for unlocking the nanoscale world.

Learn more


Continue the journey to a perfect calibration

Achieving groundbreaking insights doesn’t stop at maintaining fluorescence stability. To truly unlock the potential of super-resolution microscopy, precise calibration is key. That’s where SpheroRuler steps in, a companion innovation designed to ensure the highest accuracy in single-molecule localization microscopy (SMLM).

These meticulously engineered calibration beads provide researchers with a reliable standard, enabling them to fine-tune their imaging systems and trust every nanometer of their measurements.

 Together, EverSpark and SpheroRuler create a seamless workflow, empowering scientists to explore the nanoscale world with unparalleled clarity and confidence. 

Discover more