BiographyI was born in Algiers in 1969. I followed a biological cursus at Grenoble University and then chose a lab mixing computing sciences and microscopy imaging for my Ph.D with Michel Robert-Nicoud on genome mapping by fluorescence in situ hybridization obtained in 1997. Attracted by live-cell imaging, I decided to join the Kevin Sullivan lab at TSRI in San Diego to work on centromeres and kinetochores. In 2004, I was selected to start my junior group at ENS Lyon on Epigenetics of pericentromeres. In 2007, I moved to a recent onsite transdisciplinary lab to work with chemists and physicists, where I was recruited as research engineer at CNRS in 2009. I started to work with Philippe Bouvet's group on nucleolin and jointly developed a new project on super-resolution microscopy with a transdisciplinary consortium in the Joliot Curie Lab.
Research topicsCurrently I work at CRCL (Centre de Recherche en Cancérologie de Lyon), in
Jean-Jacques Diaz's team on:
1- Pursuing the development of Everspark buffer for single molecule
localisation microscopy for multi-color imaging applications.
2- Positionning the essential protein nucleolin in the centrosomal space
and basal body space to better decipher its function at centrosome
before and after ciliogenesis.
Fields of interest
Cellular functions of nucleolin, positionning of nucleolin at centrosome, cell surface regulation of nucleolin in cancer cells, centromeres and kinetochores in mitosis, pericentromeres and heterochromatin in S and G2 phases.
Several publicationsUgrinova I, Chalabi-Dchar M, Monier K, Bouvet P. Nucleolin interacts and co-localizes with components of pre-catalytis splicesome complexes. Sci 2019, 1, 33; https://www.mdpi.com/2413-4155/1/2/33
Duret D, Haftek-Terreau Z, Carretier M, Berki T, Ladavière C, Monier K, Bouvet P, Marvel J, Leverrier Y, Charreyre M.T, Favier A. Labeling of native proteins with fluorescent RAFT polymer probes: application to the detection of a cell surface protein using flow cytometry. 2018 9,1857. c https://pubs.rsc.org/en/content/articlelanding/2018/py/c7py02064c#!divAbstract
Kumar S, Gomez EC, Chalabi-Dchar M, Rong C, Das S, Ugrinova I, Gaume X, Monier K, Mongelard F, Bouvet P. Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin. Sci Rep. 2017 Aug 21;7(1):9017. https://pubmed.ncbi.nlm.nih.gov/28827664
Gaume X, Place C, Delage H, Mongelard F, Monier K, Bouvet P. Expression of Nucleolin Affects Microtubule Dynamics. PLoS One. 2016 Jun 16;11(6):e0157534. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911123/
Gaume X, Tassin AM, Ugrinova I, Mongelard F, Monier K, Bouvet P. Centrosomal nucleolin is required for microtubule network organization. Cell Cycle. 2015;14(6):902-19. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614815/
Monier K, Mouradian S, Sullivan KF. DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains. J Cell Sci. 2007 Jan 1;120(Pt 1):101-14. Epub 2006 Dec 12. https://jcs.biologists.org/content/120/1/101.long
Ugrinova I, Monier K, Ivaldi C, Thiry M, Storck S, Mongelard F, Bouvet P. Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication. BMC Mol Biol. 2007 Aug 10;8:66. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976620/
Monier K, Heliot L, Rougeulle C, Heard E, Robert-Nicoud M, Vourc'h C, Bensimon A, Usson Y. Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study. Cytogenet Cell Genet. 2001;92(1-2):59-62. https://www.karger.com/Article/Abstract/56869
Monier K, Armas JC, Etteldorf S, Ghazal P, Sullivan KF. Annexation of the interchromosomal space during viral infection. Nat Cell Biol. 2000 Sep;2(9):661-5. https://www.nature.com/articles/ncb0900_661
Shelby RD, Monier K, Sullivan KF. Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol. 2000 Nov 27;151(5):1113-8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174364/