Ludovic JULLIEN
Paris
"Introducing chemical concepts and technologies to overcome limitations in questioning living matter"
Biography
I have been trained as a chemist at Ecole Normale Supérieure (ENS, Paris) and Université Pierre et Marie Curie (UPMC, Paris). After my PhD in Supramolecular Chemistry and Photophysics (Prof. Jean-Marie Lehn, Collège de France, and Prof. Bernard Valeur, Conservatoire National des Arts et Métiers, Paris) and my post-doc in Soft Matter and Biophysics (Prof. Helmut Ringsdorf, Mainz, and Prof. Erick Sackmann Münich, Germany), I became Research Assistant at CNRS (Collège de France) and then Professor at Sorbonne Université and ENS.Research topics
My culture is at the triple interface between Chemistry, Biology, and Physics. My research activity has always been motivated and inspired by the amazing properties of living matter. Leading experimental, theoretical, and instrumental developments, I have correspondingly introduced various chemical tools for quantitative dynamic descriptions in Biology and conversely interrogated dynamic biological phenomena with the perspective of applications in Chemistry.Fields of interest
Systems Chemistry, Supramolecular Chemistry, Biophysical Chemistry, Analytical Chemistry, Thermokinetics.
Technologies
Applications
Actiflash
Actiflash is the stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination.
Several publications
Quérard J, Zhang R, Kelemen Z, Plamont MA, Xie X, Chouket R, Roemgens I, Korepina Y, Albright S, Ipendey E, Volovitch M, Sladitschek HL, Neveu P, Gissot L, Gautier A, Faure JD, Croquette V, Le Saux T, Jullien L. Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations. NatCommun. 2017 Oct 17;8(1):969. https://www.nature.com/articles/s41467-017-00847-3Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht CG, Shi J, Quérard J, Pan B, Rossignol J, Moncoq K, Morellet N, Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien L, Gautier A. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. https://www.pnas.org/content/113/3/497.long
Querard J, Markus TZ, Plamont MA, Gauron C, Wang P, Espagne A, Volovitch M, Vriz S, Croquette V, Gautier A, Le Saux T, Jullien L. Photoswitching kinetics and phase-sensitive detection add discriminative dimensions for selective fluorescence imaging. Angew Chem Int Ed Engl. 2015 Feb 23;54(9):2633-7. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201408985
Baffou G, Rigneault H, Marguet D, Jullien L. A critique of methods for temperature imaging in single cells. Nat Methods. 2014 Sep;11(9):899-901. https://www.nature.com/articles/nmeth.3073
Fournier L, Gauron C, Xu L, Aujard I, Le Saux T, Gagey-Eilstein N, Maurin S, Dubruille S, Baudin JB, Bensimon D, Volovitch M, Vriz S, Jullien L. A blue-absorbing photolabile protecting group for in vivo chromatically orthogonal photoactivation. ACS Chem Biol. 2013 Jul 19;8(7):1528-36. https://pubs.acs.org/doi/10.1021/cb400178m
Emond M, Le Saux T, Allemand JF, Pelupessy P, Plasson R, Jullien L. Energy propagation through a protometabolism leading to the local emergence of singular stationary concentration profiles. Chemistry. 2012 Nov 5;18(45):14375-83. https://www.ncbi.nlm.nih.gov/pubmed/23011974
Plasson R, Brandenburg A, Jullien L, Bersini H. Autocatalyses. J Phys Chem A. 2011 Jul 1;115(28):8073-85. https://pubs.acs.org/doi/10.1021/jp110079p
Zrelli K, Barilero T, Cavatore E, Berthoumieux H, Le Saux T, Croquette V, Lemarchand A, Gosse C, Jullien L. Temperature modulation and quadrature detection for selective titration of two-state exchanging reactants. Anal Chem. 2011 Apr 1;83(7):2476-84. https://pubs.acs.org/doi/10.1021/ac1026034
Sinha DK, Neveu P, Gagey N, Aujard I, Benbrahim-Bouzidi C, Le Saux T, Rampon C, Gauron C, Goetz B, Dubruille S, Baaden M, Volovitch M, Bensimon D, Vriz S, Jullien L. Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination. Chembiochem. 2010 Mar 22;11(5):653-63. https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201000008
Charier S, Meglio A, Alcor D, Cogné-Laage E, Allemand JF, Jullien L, Lemarchand A. Reactant concentrations from fluorescence correlation spectroscopy with tailored fluorescent probes. An example of local calibration-free pH measurement. J Am Chem Soc. 2005 Nov 9;127(44):15491-505. https://pubs.acs.org/doi/10.1021/ja053909w