Mayeul COLLOT
Université de Strasbourg
« I wish I could be as bright as the probes I develop »
Biography
"I started studying biology and chemistry at the Pierre and Marie Curie University (Paris VI), where I finally graduated in molecular chemistry. I started my PhD at the Ecole Normale Supérieure (ENS) in Paris in 2004 on the medicinal chemistry of carbohydrates, especially in the synthesis of oligosaccharides. In 2008, I joined Prof. Seeberger's group as a postdoctoral fellow (ETH Zurich and Max Planck Institute Berlin) where I worked on the automated synthesis of Glycocsaminoglycans and on multivalent carbohydrate systems. In 2010, I returned to Paris where I changed the subject of research and started to develop fluorescent probes and nanostructured sensors. In 2013, I was recruited at the National Center for Scientific Research (CNRS) where I currently work at UMR-7021 (Strasbourg) and where I develop fluorescent and fluorogenic tools for bio-imaging."
Lipilight by MemBright
MemBright has been designed to ensure efficient, fast, homogeneous and bright fluorescence staining of cell plasma membranes. MemBright is compatible with various samples, including 2D and 3D cell cultures, organoids and tissues.
Enable precise, reliable calibration for dSTORM microscopy with:
SpheroRuler: Calibration beads for super-resolution microscopy
Ensure prolonged, stable blinking for microscopy with:
Everspark - A long-lasting buffer for dSTORM
Several publications
Collot M, Ashokkumar P, Anton H, Boutant E, Faklaris O, Galli T, Mély Y, Danglot L, Klymchenko AS. MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Cell Chem Biol. 2019 Apr 18;26(4):600-614.e7. https://doi.org/10.1016/j.chembiol.2019.01.009Collot M, Boutant E, Lehmann M, Klymchenko AS. BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe. Bioconjug Chem. 2019 Jan 16;30(1):192-199. https://doi.org/10.1021/acs.bioconjchem.8b00828
Collot M, Fam TK, Ashokkumar P, Faklaris O, Galli T, Danglot L, Klymchenko AS.Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues. J Am Chem Soc. 2018 Apr 25;140(16):5401-5411. https://doi.org/10.1021/jacs.7b12817
Collot M, Bou S, Fam TK, Richert L, Mély Y, Danglot L, Klymchenko AS. Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push-Pull Fluorophore. Anal Chem. 2019 Feb 5;91(3):1928-1935. https://doi.org/10.1021/acs.analchem.8b04218
Despras G, Zamaleeva AI, Dardevet L, Tisseyre C, Magalhaes JG, Garner C, De Waard M, Amigorena S, Feltz A, Mallet JM, Collot M. H-Rubies, a new family of red emitting fluorescent pH sensors for living cells. Chem Sci. 2015 Oct 1;6(10):5928-5937. https://doi.org/10.1039/C5SC01113B
Ponsot F, Shen W, Ashokkumar P, Audinat E, Klymchenko AS, Collot M. PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience. ACS Sens. 2017 Nov 22;2(11):1706-1712. https://doi.org/10.1021/acssensors.7b00665
Collot M, Ponsot F, Klymchenko AS. Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore. Chem Commun (Camb). 2017 Jun 1;53(45):6117-6120. https://doi.org/10.1039/C7CC02418E
Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM. CaRuby-Nano: a novel high affinity calcium probe for dual color imaging. Elife. 2015 Mar 31;4. https://doi.org/10.7554/eLife.05808
Collot M, Loukou C, Yakovlev AV, Wilms CD, Li D, Evrard A, Zamaleeva A, Bourdieu L, Léger JF, Ropert N, Eilers J, Oheim M, Feltz A, Mallet JM. Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. J Am Chem Soc. 2012 Sep 12;134(36):14923-31. https://doi.org/10.1021/ja304018d