« I wish I could be as bright as the probes I develop »
BiographyI started studying biology and chemistry at the Pierre and Marie Curie University (Paris VI), where I finally graduated in molecular chemistry. I started my PhD at the Ecole Normale Supérieure (ENS) in Paris in 2004 on the medicinal chemistry of carbohydrates, especially in the synthesis of oligosaccharides. In 2008, I joined Prof. Seeberger's group as a postdoctoral fellow (ETH Zurich and Max Planck Institute Berlin) where I worked on the automated synthesis of Glycocsaminoglycans and on multivalent carbohydrate systems. In 2010, I returned to Paris where I changed the subject of research and started to develop fluorescent probes and nanostructured sensors. In 2013, I was recruited at the National Center for Scientific Research (CNRS) where I currently work at UMR-7021 (Strasbourg) and where I develop fluorescent and fluorogenic tools for bio-imaging.
Research TopicsMy research focuses on the development of molecular and nanostructured fluorescent probes for bio-imaging. Our probes are based on different systems providing effective fluorescent ignition effects or spectral modifications when detecting cellular constituents (plasma membrane, lipid droplets) or cellular events (calcium detection, pH variation, detection of reducing / oxidizing environment). Our goal is to develop brightt, versatile fluorophores that can be used in both multiphoton and super-resolution microscopy. I am also working on fluorescent and very bright nanoemulsions and small polymer nanoparticles that can be easily functionalized to develop fluorescent nanobiosensors.
Fields of interest
Development of fluorescent tools; Fluorogenic probes; Fluorescent nanoparticles; Bioimaging
Organelle-specific probes; Calcium probes; PH probes
Plasma membrane; mitochondria; Lipid droplets
Multiphoton imaging; Super-resolution imaging Confocal microscopy
MemBright has been designed to ensure efficient, fast, homogeneous and bright fluorescence staining of cell plasma membranes. MemBright is compatible with various samples, including 2D and 3D cell cultures, organoids and tissues.
Several publicationsCollot M, Ashokkumar P, Anton H, Boutant E, Faklaris O, Galli T, Mély Y, Danglot L, Klymchenko AS. MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Cell Chem Biol. 2019 Apr 18;26(4):600-614.e7. https://doi.org/10.1016/j.chembiol.2019.01.009
Collot M, Boutant E, Lehmann M, Klymchenko AS. BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe. Bioconjug Chem. 2019 Jan 16;30(1):192-199. https://doi.org/10.1021/acs.bioconjchem.8b00828
Collot M, Fam TK, Ashokkumar P, Faklaris O, Galli T, Danglot L, Klymchenko AS.Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues. J Am Chem Soc. 2018 Apr 25;140(16):5401-5411. https://doi.org/10.1021/jacs.7b12817
Collot M, Bou S, Fam TK, Richert L, Mély Y, Danglot L, Klymchenko AS. Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push-Pull Fluorophore. Anal Chem. 2019 Feb 5;91(3):1928-1935. https://doi.org/10.1021/acs.analchem.8b04218
Despras G, Zamaleeva AI, Dardevet L, Tisseyre C, Magalhaes JG, Garner C, De Waard M, Amigorena S, Feltz A, Mallet JM, Collot M. H-Rubies, a new family of red emitting fluorescent pH sensors for living cells. Chem Sci. 2015 Oct 1;6(10):5928-5937. https://doi.org/10.1039/C5SC01113B
Ponsot F, Shen W, Ashokkumar P, Audinat E, Klymchenko AS, Collot M. PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience. ACS Sens. 2017 Nov 22;2(11):1706-1712. https://doi.org/10.1021/acssensors.7b00665
Collot M, Ponsot F, Klymchenko AS. Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore. Chem Commun (Camb). 2017 Jun 1;53(45):6117-6120. https://doi.org/10.1039/C7CC02418E
Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM. CaRuby-Nano: a novel high affinity calcium probe for dual color imaging. Elife. 2015 Mar 31;4. https://doi.org/10.7554/eLife.05808
Collot M, Loukou C, Yakovlev AV, Wilms CD, Li D, Evrard A, Zamaleeva A, Bourdieu L, Léger JF, Ropert N, Eilers J, Oheim M, Feltz A, Mallet JM. Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. J Am Chem Soc. 2012 Sep 12;134(36):14923-31. https://doi.org/10.1021/ja304018d