FlashFix - Optofixation solution
Solution for cell fixation with high spatial and temporal resolution.
A technology originally developed by Stephanie Bosch (1), Maelle Carraz (1,2), Romain Duval (2)
(1) Centre de Biologie Intégrative (CBI), Unité de Biologie Moléculaire, Cellulaire et du Développement (MCD), CNRS, Toulouse, France
(2) Institut de Recherche pour le Développement (IRD), Paris , France
FlashFix, a photofixative technology.
FlashFix is a new way of fixing cell with high spatial and temporal resolution. It is based on a molecule which can trigger cell fixation when exposed to light in the proper culture conditions.
Fast fixation
Cells are snap fixed, down to 7 seconds
Precision
Resolution of fixation down to single cell
Visible
Nuclei are stained in green after photofixation
How does FlashFix work ?
Applications
- Snap fix the cells at a precise point to capture rare cellular events.
- Study the behaviour of surrounding cells when a group of cells are fixed, similarly to photo-ablation
Validated samples
FlashFix has been successfully used on a variety of cells :
- Hep3B : liver cancer cell line
- C4-2B : prostate cancer cell line
- PC3 : bone metastasis cell line of a grade IV prostatic adenocarcinoma
- T24 : urinary bladder cell line of colorectal adenocarcinoma
- WI-38 : fibroblast-like fetal lung cell.
- Cells dissociated from zebrafish embryo
Kit description:
- 1000 X stock photofixing solution
- Specific cell culture media