StrigoLightOn - Pro-fluorescent analogs of strigolactones
Catalog of pro-fluorescent analogs of strigolactones
A technology developed by
Guillaume Clavé (Institute of Biomolecules Max Mousseron, IBMM, France)
and François-Didier Boyer (Institute of Chemistry of Natural Substances, ICSN, France)
StrigoLightOn compounds are pro-fluorescent strigolactone analogs designed to mimic diverse strigolactone structures. These variants enable deeper insights into receptor-ligand studies by generating more physiologically relevant data.
Simple and real-time tracking of strigolactone receptor activation
Due to their specific design, StrigoLightOn compounds emit fluorescence exclusively upon binding to a strigolactone receptor.
Enhanced versatility in strigolactone mimicry
The availability of three distinct StrigoLightOn variants allows for more accurate replication of natural strigolactone diversity in physiologically meaningful contexts.
Universal model to study strigolactones
StrigoLightOn were shown to bind receptors from D14 and KAI2 family and their bioactivity was tested on many plants.
Description of StrigoLightOn
We offer 3 different molecules with 0, 1 or 2 methylation site on the D-ring that fully represent the variety of strigolactones.
StrigoLightOn-486
The D-ring has no methylation site
StrigoLightOn-240
The D-ring has one methylation site
StrigoLightOn-242
The D-ring has two methylation sites
How does StrigoLightOn work ?
StrigoLightOn leverage the operational mechanism of strigolactone receptors.
Upon strigolactone binding, the receptor undergoes hydrolysis, retaining the active moiety covalently while releasing the cargo group—triggering downstream biological functions like protein recruitment.
In the case of our pro-fluorescent analogs, the released molecule becomes fluorescent upon cleavage (hydrolysis) by the receptor. This innovative approach provides a powerful tool for visualizing and studying receptor activity in real-time, offering unparalleled insights into biological processes.
Applications of StrigoLightOn
- Real time monitoring of strigolactone receptor activity through the emitted fluorescence (Previously performed with the following receptors : AtD14, RMS3, OsD14, DAD2, AtKAI2, PrKAI2d3).
- Determination of the affinity of a ligand with competition assay.
- Analysis of strigolactone activity in planta : Bio-activity of StrigoLightOn was previously tested on a variety of plants for their hormonal activity (Arabidopsis Thaliana, Pisum Sativum, Physcomitrella Patens) and parasitic plants for their germination (Pheliplanche Ramosa, Striga Hermonthica).
Kit content of StrigoLightOn
StrigoLightOn are available as 10mM concentrated solutions. To meet various experimental needs, we designed different kit sizes :
Storage and stability: Store the solution at -20°C in darkness for year.
Summary of receptor activity determined by StrigoLightOn fluorescence
Fluorescence analysis reveals variations in the response of a single StrigoLightOn across different receptors, as well as differences among multiple StrigoLightOn for a given receptor. These results highlight the importance of having a diversity of analogs, enabling a suitable choice based on the type of receptor being studied.
Data was adapted from De Saint Germain et al 2016, De Saint Germain et al 2021, De Saint Germain et al 2022.
Competition assays can be conveniently conducted by monitoring changes in fluorescence emitted by StrigoLightOn.
The shift in fluorescence from the beggining of the reaction shows variation of binding of the StrigoLightOn depending on the presence of GR24 and its concentration.
Credit : Guillaume Clavé, Institut des Biomolécules Max Mousseron, France.
StrigoLightOn exhibit strong hormonal bioactivity across multiple plant models
Pisum Sativum
Arabidopsis Thaliana
Physcomitrium (Physcomitrella) PatensFor each plant tested, there is a StrigoLightOn with bioactivity superior to Yoshimulactone Green (YLG), and/or similar to GR24, a commonly used synthetic strigolactone analog . The various tests conducted on the plants also illustrate variations in the effects of different StrigoLightOn, justifying the use of 3 different analogs that will be more or less active depending on the plant being studied.
Data was adapted from De Saint Germain et al 2016, De Saint Germain et al 2022.
StrigoLightOn can activate germination of parasitic seeds
Relative germination stimulant (GS) activity compared to that of GR24
Data was adapted from De Saint Germain et al 2016.
Germination of Pheliplanche Ramosa seeds after incubation with StrigoLightOn-242 (right) compared with control condition (left)
Credit : Jean-Bernard Pouvreau, US2B, Nantes University.