GlowMito

A ready-to-use solution for real-time imaging of mitochondria

REGISTER TO ITS TEST PROGRAM

Overview

GlowMito is a cell permeant fluorescent probe that is selective for the mitochondria. Derived from a compound with high intrinsic fluorescence, GlowMito quickly penetrates cells and produces a bright labeling of mitochondria without inducing mitotoxicity or altering cell physiology. It has been successfully used for mitochondria imaging in live cells.

GlowMito is a fantastic illustration of the fruitful discoveries that can arise when organic chemistry meets biology, and it paves the way to a new class of fluorescent probes.

A technology designed by Olivier Siri and Guy Lenaers .

The Test Program is open

Want to give GlowMito a try on your own research project? Join the Test Program!
If you're new to our Test Programs, their objectives and our engagements, discover them here.

Registrations are closing on: May 26, 2023

Number of days left before we close the registrations:

I REGISTER NOW

Images

COS-7 cells labeled with 500 nM GlowMito - Scale bar = 20µm

HeLa cells labeled with 500 nM GlowMito - Scale bar = 20µm

Features

Non-toxic

Based on weak instead of covalent interactions and diluted in water, GlowMito preserves cell survival and can be used for long-term imaging of mitochondria without washing

Ready-to-use

provided as a stable liquid solution. Simply add it to your cell culture medium, and image!

Cell physiology preserved

It does not affect mitochondrial respiration

Compatible

with one and two-photon microscopy

Protocol

Grow your cells

Dilute

GlowMito 1:2000 in your cell culture medium

Incubate

for 30 minutes at 37°C

Image

Technical information

Absorption & emission spectra of GlowMito in H²O

Colour: Red/near-infrared
Detection method : Fluorescent
Peak excitation/emission wavelengths: Ex:542nm/Em:690nm
Two-photon absorption peak: 790nm
Two-photon cross-section: 224 GM
Form : Liquid
Sub-cellular localization: mitochondria
For use with: confocal microscopes, flow cytometers

1 vial contains 125 µL of an aqueous solution with a concentration of 1 mM for a final volume of 250 mL of imaging medium
Storage: Store at -20°C protected from light

Outputs:
- Endpoint analysis : qualitative (mitochondrial structure, fusion/fission, spatial distribution) and quantitative (mitochondrial mass) assays
- Subsequent analysis: flow cytometry, oxygraphy, etc
GlowMito can be used in multiparameter analyses without fluorescence interference.

Tested and validated on: HEK293 cells, MCF-7 breast cancer cells, HeLa cells and COS-7 cells.

Safety datasheet to download here.

Story

"As a chemist, Olivier thrives on finding new synthetic routes giving access to smart molecules. In 2015, he discovered by serendipity a new chemical pathway to reach triaminophenazinium compounds. These molecules were already well known, but their synthetic route drastically limited the nature of the substituents carried by the amine functions. Therefore, Olivier decided to find applications for them in biology and one of the most promising was the mitochondrial tagging. From then on, the collaboration with Guy was obvious. As a specialist of mitochondrial medicine, he would undoubtedly contribute to furthering this marking! Their first approach in fluorescence microscopy made it possible to localize selectively the water-soluble phenaziniums at the level of the mitochondrial network. Together, they characterized the optimal concentration of each triaminophenazinium compound and their kinetics of mitochondrial labeling, further demonstrated their safety on mitochondrial respiration function. GlowMito was born!"

Olivier Siri and Guy Lenaers

Esther Graudens
New projects at Idylle

The association between Olivier and Guy is synergistic. In addition to their individual talent, it convinced us that their team would drive their tech transfer project further. Our work together was highly fluid and we managed to transfer their technology in a very short time. We are thrilled to launch the GlowMito marker on the research market and I am looking forward to getting the first feedback of use from the early-users!