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Karidia KONATE

Montpellier


Biography 

I was graduated in chemistry at Pierre and Marie Curie’ University and completed my formation with a degree in the design of products formulated from dispersed systems. In 2005, I was recruited as CNRS engineer at the Centre de Recherche de Biologie cellulaire de Montpellier (CRBM) to characterize cell penetrating peptides and their mode of action by biophysical technics. As I work in a multidisciplinary team, obtaining my PhD (2015) was a logical consequence for me in the understanding and involvement in the research project that I was implied for many years. Recently, I joined the team n°4 "Development of the visceral smooth muscle and associated pathologies" at the laboratory Physiology and Experimental Biology of Heart and Muscles (PhyMedExp).
                
                  

Research Topics

I work on the design and the development of cell penetrating peptides as a delivery system of nucleic acids but also as therapeutic adjuvant in gene therapy for disease like cancer or gastrointestinal disorders. I characterize the Peptide Based Nanoparticles (PBNs) formed by the combination of peptide and nucleic acids and evaluate their stability and their delivery efficiency in cell culture.  Membrane models (lipids bilayer as liposomes or lipids monolayer) are also a part of my expertise important in the comprehension of peptide behaviour in the delivery process at membrane level.

Fields of interest

Cell Penetrating Peptide, Nanoparticles, Formulation, Stability, Biology, Therapeutic.

Technologies

Dynamic Light Scattering, Circular Dichroïsm, Fluorescence, Membrane models (Langmuir monolayers-liposomes), Western blot, Quantification assays (luciferase, LDH, BCA, etc.), Plasmide amplification, Cell culture, Microscopy.


Applications

Cell-penetrating peptides, Nanoparticle.

WRAP

 

WRAP deliver safely nucleic acids in you favorite cells!

Several publications

Konate K, Seisel Q, Vivès E, Boisguérin P, Deshayes S. Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide. J Vis Exp. 2020 Dec 19;(166). 


Deshayes S, Konate K, Dussot M, Chavey B, Vaissière A, Van TNN, Aldrian G, Padari K, Pooga M, Vivès E, Boisguérin P. Deciphering the internalization mechanism of WRAP:siRNA nanoparticles. Biochim Biophys Acta Biomembr. 2020 Jun 1;1862(6):183252.

Sousa Â, Almeida AM, Faria R, Konate K, Boisguerin P, Queiroz JA, Costa D. Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments. Colloids Surf B Biointerfaces. 2019 Nov 1;183:110417.

 Konate K, Dussot M, Aldrian G, Vaissière A, Viguier V, Neira IF, Couillaud F, Vivès E, Boisguerin P, Deshayes S. Peptide-Based Nanoparticles to Rapidly and Efficiently "Wrap 'n Roll" siRNA into Cells. Bioconjug Chem. 2019 Mar 20;30(3):592-603.

Vaissière A, Aldrian G, Konate K, Lindberg MF, Jourdan C, Telmar A, Seisel Q, Fernandez F, Viguier V, Genevois C, Couillaud F, Boisguerin P, Deshayes S. A retro-inverso cell-penetrating peptide for siRNA delivery. J Nanobiotechnology. 2017 Apr 28;15(1):34.

Aldrian G, Vaissière A, Konate K, Seisel Q, Vivès E, Fernandez F, Viguier V, Genevois C, Couillaud F, Démèné H, Aggad D, Covinhes A, Barrère-Lemaire S, Deshayes S, Boisguerin P. PEGylation rate influences peptide-based nanoparticles mediated siRNA delivery in vitro and in vivo. J Control Release. 2017 Jun 28;256:79-91. doi: 10.1016/j.jconrel.2017.04.012.

Konate K, Lindberg MF, Vaissiere A, Jourdan C, Aldrian G, Margeat E, Deshayes S, Boisguerin P. Optimisation of vectorisation property: A comparative study for a secondary amphipathic peptide. Int J Pharm. 2016 Jul 25;509(1-2):71-84. 

Konate K, Rydstrom A, Divita G, Deshayes S. Everything you always wanted to know about CADY-mediated siRNA delivery* (* but afraid to ask). Curr Pharm Des. 2013;19(16):2869-77. doi: 10.2174/1381612811319160004. PMID: 23140452 Review.

Deshayes S, Konate K, Rydström A, Crombez L, Godefroy C, Milhiet PE, Thomas A, Brasseur R, Aldrian G, Heitz F, Muñoz-Morris MA, Devoisselle JM, Divita G. Self-assembling peptide-based nanoparticles for siRNA delivery in primary cell lines. Small. 2012 Jul 23;8(14):2184-8.

Olugbile S, Villard V, Bertholet S, Jafarshad A, Kulangara C, Roussilhon C, Frank G, Agak GW, Felger I, Nebie I, Konate K, Kajava AV, Schuck P, Druilhe P, Spertini F, Corradin G. Malaria vaccine candidate: design of a multivalent subunit α-helical coiled coil poly-epitope. Vaccine. 2011 Sep 16;29(40):7090-9.