Lipilight cell membrane fluorescent probe

Lipilight

powered by the MemBright technology

 

  

Designed by Andrey Klymchenko, Mayeul Collot

"We designed MemBright with the idea to make fluorescence staining of cell plasma membranes simple and efficient. These probes are compatible with practically any fluorescence imaging technique, from basic “everyday” microscopy up to advanced super-resolution imaging. MemBright probes are available in five standard colors, providing a fast, bright and homogeneous staining at low concentration and with reduced background noise. We hope you will enjoy these probes as much as we did while developing them!"  

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    EXAMPLES OF RESULTS

    APPLICATIONS

     TECHNOLOGY FEATURES

     METHODS

    USERS FEEDBACKS

    PUBLICATIONS

  EXAMPLES OF RESULTS

MemBright has been designed to ensure efficient, fast, homogeneous and bright staining of cell plasma membranes. MemBright is compatible with various samples, including 2D and 3D cell cultures, organoids and tissues.

Odoo • Image et Texte
Plasma membrane imaging of hippocampal neurons labeled with MemBright-590 technology using dSTORM microscopy. Widefield image is in fire LUT, light and dark blue spheres correspond to single molecule detection of MemBright-590 within the  plasma membrane. Depth is color coded from cyan to dark blue.
Image credit: @Lydia Danglot
Odoo • Image et Texte
Laser scanning confocal microscopy of seeded KB cells stained using MemBright-488 technology at 20nM.
Image credit: @Mayeul Collot 
Odoo • Image et Texte
Laser scanning confocal microscopy of live KB cells stained using MemBright technology at 20 nM.
Image credit: @Mayeul Collot

  APPLICATIONS

Domains:

  • 2D and 3D cell culture
  • Exosomes
  • Tissues

Techniques:

  • Epi-fluorescence
  • Confocal imaging
  • Super-resolution (dSTORM)
  • Bi-photon microscopy

  TECHNOLOGY FEATURES

Versatility: compatible with liposomes, exosomes, apoptotic bodies, living and fixed cells, spheroids, organoids, tissues, etc.

5 colors: emission from 500 nm to 700 nm.

Bright and photostable: used at low concentrations of 20 to 200 nM for cells in culture. Suitable for long-term imaging

Fast staining: 2-15 min

Reduced tocxicity: The brightness of MemBright allows it to be used at low concentrations (as low as 10 nM) with low excitation power.

  METHODS

Storage conditions

  • Short-term preservation: at + 4 ° C protected from light
  • Long-term storage: at -20 ° C protected from light

Protocol of use

For adherent cells:

  • Before staining, prepare a solution of MemBright in your visualization medium such as PBS, HBSS, MEM, Opti-MEM, Krebs, etc. (Caution: do not use medium with serum) with a final concentration ranging from 10 nM to 200 nM.
  • Remove the sample from its support, wash the sample twice (for example with PBS) and cover your cells with the freshly prepared MemBright solution (do not store this solution for more than a minute without cells).
  • No need to wash the cells of the dye, visualize directly.
  • Alternatively, it is also possible to add a small volume of a diluted solution of MemBright (4 to 40 μM) directly into the middle of your sample, then mix gently.
All MemBright stock solutions are prepared in DMSO.

  USERS FEEDBACKS

  PUBLICATIONS

Collot M, Ashokkumar P, Anton H, Boutant E, Faklaris O, Galli T, Mély Y, Danglot L, Klymchenko AS. MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Cell Chem Biol. 2019 Apr 18;26(4):600-614.e7. https://doi.org/10.1016/j.chembiol.2019.01.009

Hyenne V, Ghoroghi S, Collot M, Bons J, Follain G, Harlepp S, Mary B, Bauer J,Mercier L, Busnelli I, Lefebvre O, Fekonja N, Garcia-Leon MJ, Machado P, Delalande F, López AA, Silva SG, Verweij FJ, van Niel G, Djouad F, Peinado H,Carapito C, Klymchenko AS, Goetz JG. Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo. Dev Cell.2019 Feb 25;48(4):554-572.e7.https://doi.org/10.1101/380238

Collot M, Boutant E, Lehmann M, Klymchenko AS. BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe. Bioconjug Chem. 2019 Jan 16;30(1):192-199. https://doi.org/10.1021/acs.bioconjchem.8b00828

Collot M, Fam TK, Ashokkumar P, Faklaris O, Galli T, Danglot L, Klymchenko AS.Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues. J Am Chem Soc. 2018 Apr 25;140(16):5401-5411. https://doi.org/10.1021/jacs.7b12817

Ponsot F, Shen W, Ashokkumar P, Audinat E, Klymchenko AS, Collot M. PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience. ACS Sens. 2017 Nov 22;2(11):1706-1712. https://doi.org/10.1021/acssensors.7b00665