Stampwell V - 3D cell imaging
Mold agarose wells to parallelize spheroid and organoid imaging
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Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids and organoids.
Two different shapes of Stampwell V
V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
Additional resources:
Imprinted V-shape well in agarose, using Stampwell V-300
Soaked in fluorescein, negative rendering
Image credit: Gaëlle Recher - Bordeaux
hiPSCs cyst in alginate capsule in a V-shape well made with Stampwell V
scale bar: 500µm
Image credit: Gaëlle Recher - Bordeaux
Cell-filled alginate capsule in a well molded with Stampwell V
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
V-shape imprinted wells, using Stampwell V
Filled with a single cell-loaded alginate capsule: corresponding imaged with a stereomicroscope and stitched. Gel pad is made of 2% agarose. (Scale bar: 2mm)
Image credit: Gaëlle Recher - Bordeaux
Apical-out 10-day human airway organoids imaged in a V-shape Stampwell well in comparison with suspension plates
Airway organoids were generated from a human bronchial cell line in hydrogel and transferred either to microwells made using a V-shape Stampwell, or suspension plates, for turning them inside out and imaging.
Credits: Signe Lolle - Danmarks Tekniske Universitet, Denmark
How to use Stampwell V:
Protocol overview
1. Pour liquid agarose (or other hydrogel)
2. Place the stamp
3. Reticulate the hydrogel
4. Remove the stamp
"The Stampwell family of products is born from a technology that I designed, called Mount(n). It is the result of my expertise in imaging a wide range of 3D thick samples. I navigated through many communities (gathered around a biological model), and each time I learnt new tricks to mount samples for microscopy experiments. The Mount(n) technology consists in imprinting an array of wells into a hydrogel (typically agarose), each well receiving a 3D sample (embryos of any species, organoids, tissue biopsies...). The design of the wells pr events the object from moving when the plate or dish is moved, does not apply any mechanical constraints to the sample, and paradoxically also enables the sample to keep growing inside. In a single dish, tens of samples can be imaged with any microscope, for as long as necessary. "
Esther Graudens,
Academic partenerships manager at Idylle
" Together, we turned the Mount(n) technology into a family of research products. First, we have evolved the shape, the color and the material to offer an ergonomic grip and functionalities that meet the usage characteristics of most applications. Then, as we were about to start the production, the first testers found out that the stamp did not fit in all their dishes. Within a few days, together with Gaëlle and our design office, we redesigned a shape and in April 2021, the Test Program for the first Stampwell models, 2 V-shapes and Embryo 1, was released.
But the story does not end up here! More and more researchers asked us to customize a stamp to image zebrafish and medaka embryos or larvae. So in addition to the Embyo 1, at Idylle we decided to add 3 new models that would facilite imaging them up to 20 dpf.
The rest of the story remains to be written with you!"
I successfully managed to use Stampwell with Collagen-1.
I use the Stampwell V-shape to track the explants development and the embryoids formation from stem cell aggregates in zebrafish, and it makes my work much easier!
Utilizing the Stampwell V-shape when turning airway organoids inside out is useful to keep them separated, therefore avoiding clumping and merging.