Need something more tailored to your needs?
Whether it's adjusting well size, shape, or layout, we’d be delighted to work with you to bring your ideas to life.
A technology developed by Gaëlle Recher (CNRS - Université de Bordeaux, France)
Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of fish embryos or larvae up to 20 dpf.
Save time: no need to manually position your samples individually. Fish embryos/larvae will self-position in the wells.
Boost your assay reproducibility: once loaded in the wells, your samples are perfectly positioned, aligned and located on a similar focal plane.
Open system: direct access to embryos/larvae for adding external compounds (i.e. drug screening).
4 different shapes of Stampwell Z for zebrafish imaging
depending on the size and orientation of your fish embryos.
Embryo 1 (ie Rectangular)
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm
More information on the Datasheet.
Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm
More information on the Datasheet Embryo 2.
Larvae 1:
. Number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail
More information on the Datasheet Larvae 1.
Larvae 2:
. Number of pins: 5 large wells ideal for the 15-20 dpf zebrafish or 39-42 dpf medaka larvae + 5 small wells ideal for the 1-2 dpf zebrafish
. Shape of the pins: prism
. Length of the 5 large wells: 10 mm
. Length of the 5 small wells: 3 mm
. Well depth: 1 mm
More information on the Datasheet Larvae 2.
Plate compatibility:
The stamp has a total diameter of 26 mm. It can be used in:
- 35 mm circular petri dishes with an aperture of at least 22 mm
- any larger vessel (i.e. 6-well plates)
Additional resources:
Imprinted V-shape well in agarose, thanks to Stampwell V-300
Soaked in fluorescein, negative rendering
Image credit: Gaëlle Recher - Bordeaux
hiPSCs cyst in alginate capsule in a V-shape well made with Stampwell
scale bar: 500µm
Image credit: Gaëlle Recher - Bordeaux
Cell-filled alginate capsule in the V-shape well
Notice that the focus moving through the well height is easily visible (lines in focus Vs out of focus)
V-shape imprinted wells
Filled with a single cell-loaded alginate capsule: corresponding imaged with a stereomicroscope and stitched. Gel pad is made of 2% agarose. (Scale bar: 2mm)
Image credit: Gaëlle Recher - Bordeaux
How to use Stampwell Z for zebrafish imaging:
Protocol overview
1. Pour liquid agarose (or other hydrogel)
2. Place the stamp
3. Reticulate the hydrogel
4. Remove the stamp
Stampwell has been originally developed by Gaelle Recher and published in Scientific Reports:
Alessandri K, Andrique L, Feyeux M, Bikfalvi A, Nassoy P, Recher G. All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide. Sci Rep. 2017 Feb 10;7:42378. doi: 10.1038/srep42378. PMID: 28186188; PMCID: PMC5301227.
Citations:
Merdrignac C, Clément AE, Montfort J, Murat F, Bobe J. auts2 Features and Expression Are Highly Conserved during Evolution Despite Different Evolutionary Fates Following Whole Genome Duplication. Cells. 2022 Aug 30;11(17):2694. doi: 10.3390/cells11172694. PMID: 36078102; PMCID: PMC9454499.
Stampwell has proven to be a very easy to use mounting medium for confocal imaging of fixed and immunostained zebrafish embryos.
I used a Stampwell to ensure that my zebrafish embryos were positioned correctly under the microscope for spatial and temporal experiments. I am pleased with my Stampwell because it saves me time and provides precision!