The super-resolution microscopy buffer that offers multicolor, long-term and stable fluorescence imaging:
prepare your samples one day, and image them every day for weeks


Odoo • Image et Texte

No more rushing your imaging. Prepare your slide, and image quietly whenever you want, even up to 10 weeks later.
Image with red, far red and green dyes (eg. AF488). Use different colors either at the same time or one after the other.

A technology designed by Karine Monier, Arnaud Favier and  Christophe Place
Powered by  the Eternity technology published in  Scientific Reports  

"We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging"
Karine Monier, Arnaud Favier and Christophe Place
John DOE • CEO of MyCompany


Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells 

Martina RuglioniSimone CivitaTiziano SalvadoriSofia CristianiVittoria CarnicelliSerena BarachiniIacopo PetriniIrene NepitaMarco CastelloAlberto DiasproPaolo BianchiniBarbara StortiRanieri BizzarriStefano Fogli and Romano Danesi   bioRxiv 2022.08.09.503318; doi: https://doi.org/10.1101/2022.08.09.503318

HIV-1 diverts actin debranching mechanisms for particle assembly and release in CD4 T lymphocytes
Rayane Dibsy, Erwan Bremaud, Johnson Mak, Cyril Favard, Delphine Muriaux
BioRxiv, December 16, 2022. doi. 10.1101/2022.12.15.520580

Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin
Fabre L, Rousset C, Monier K, Da Cruz-Boisson F, Bouvet P, Charreyre MT, Delair T, Fleury E, Favier A. Biomacromolecules. 2022 May 12. doi: 10.1021/acs.biomac.1c01706. PMID: 35549176

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U. Haematologica. 2022 Apr 1;107(4):947-957. doi: 10.3324/haematol.2021.280154. PMID: 35045692

Metabolic biorthogonal labeling and dSTORM imaging of peptidoglycan synthesis in Streptococcus pneumoniae 
Jennyfer Trouve, Oleksandr Glushonkov and Cecile Morlot
Star Protocols, December 13, 2021. doi: 10.1016/j.xpro.2021.101006

Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia.
Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Völker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T. 
Blood. 2021 Dec 2;138(22):2256-2268. doi: 10.1182/blood.2021013231. PMID: 34587242

Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres.
Szikora S, Novák T, Gajdos T, Erdélyi M, Mihály J. Bio Protoc. 2020 Jun 20;10(12):e3654. doi: 10.21769/BioProtoc.3654. eCollection 2020 Jun 20. PMID: 33659324

Feedback from users

Everspark works with thick samples of 100µ (peripheral blinking). 

Everspark does not work 100% efficiently with organs and tissues because there is most of the times a bit of remaining oxygen.

Feedback by Randy Stout

Everspark is a game changer: it is very, very useful for anyone doing dSTORM.

Randy StoutNew York Institute of Technology, 2023

 Feedback diary




High blinking stability over time for repeated measurements

Once mounted, the sample is stable for up to 5 weeks and can be imaged multiple times

Multicolor (incl. green)

Compatible with green, red & far red dyes (JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5 and mEos2)

Ready to use​

Can be used directly from the vial

Up to 6 months performance

Individualized packaging for optimal longevity


HILO, TIRF pattern


The protocol of use with a chambered coverslip
Sealing of all chambers with the lid


The protocol of use with a chambered coverslip
Sealing half of the chambers with a coverslip


The protocol of use with a glass-bottom dish


The protocol of use with a depression slide


 A simplified p​rotocol

Recipe to prepare a good sealing


Technical information

Kit description:

  • 10 vials with 450 µL of Everspark buffer at 100 mM MEA in Tris.
  • 1 vial per experiment.

For use with: round glass-bottom dishes, standard or chambered glass coverslips.

Up to 6 months (2 months after opening the pouch + 4 months storage in the pouch).



"We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging. Indeed, an oxygen-free buffer containing a thiol reducer is central to promote fluorophore blinking and thus single molecule detection, required for dSTORM imaging. With classical buffers, such as the Glox buffer, fluorophore blinking lifetime is limited to a few hours. With Everspark buffer, it is now possible to increase sample lifetime to several weeks. We demonstrated that a calibrated sample mounted once with the Everspark buffer can be submitted to several rounds of dSTORM imaging to acquire and reconstruct a structure reproducible in coverage and precision, over a period of two months." 

Odoo • Image and Text Esther Graudens 
New projects at Idylle

"When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that's exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it".

Combining Everspark

You may also like to combine Everspark with these R&D products


Stencell will help during the sealing step when mounting your sample


Combine Everspark with SpheroRuler and get a ready-to-use kit for dSTORM calibration

Contact us about your sample preparation workflow