Overview

No more rushing your imaging. Prepare your slide, and image quietly whenever you want, even up to 10 weeks later.
Image with red, far red and green dyes (eg. AF488). Use different colors either at the same time or one after the other.
A technology designed by
Karine Monier, Arnaud Favier and Christophe Place
Powered by
the Eternity technology published in
Scientific Reports
"We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging"Karine Monier, Arnaud Favier and Christophe Place
Citations
Martina Ruglioni, Simone Civita, Tiziano Salvadori, Sofia Cristiani, Vittoria Carnicelli, Serena Barachini,
Iacopo Petrini, Irene Nepita, Marco Castello, Alberto Diaspro, Paolo Bianchini, Barbara Storti, Ranieri Bizzarri,
Stefano Fogli and Romano Danesi
bioRxiv 2022.08.09.503318; doi: https://doi.org/10.1101/2022.08.09.503318
HIV-1 diverts actin debranching mechanisms for particle assembly and release in CD4 T lymphocytes
Rayane Dibsy, Erwan Bremaud, Johnson Mak, Cyril Favard, Delphine Muriaux
BioRxiv, December 16, 2022. doi. 10.1101/2022.12.15.520580
Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin
Fabre L, Rousset C, Monier K, Da Cruz-Boisson F, Bouvet P, Charreyre MT, Delair T, Fleury E, Favier A. Biomacromolecules. 2022 May 12. doi: 10.1021/acs.biomac.1c01706. PMID: 35549176
Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U. Haematologica. 2022 Apr 1;107(4):947-957. doi: 10.3324/haematol.2021.280154. PMID: 35045692
Metabolic biorthogonal labeling and dSTORM imaging of peptidoglycan synthesis in Streptococcus pneumoniae
Jennyfer Trouve, Oleksandr Glushonkov and Cecile Morlot
Star Protocols, December 13, 2021. doi: 10.1016/j.xpro.2021.101006
Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia.
Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Völker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T.
Blood. 2021 Dec 2;138(22):2256-2268. doi: 10.1182/blood.2021013231. PMID: 34587242
Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres.
Szikora S, Novák T, Gajdos T, Erdélyi M, Mihály J. Bio Protoc. 2020 Jun 20;10(12):e3654. doi: 10.21769/BioProtoc.3654. eCollection 2020 Jun 20. PMID: 33659324
Feedback from users
Everspark works with thick samples of 100µ (peripheral blinking).
Everspark does not work 100% efficiently with organs and tissues because there is most of the times a bit of remaining oxygen.
Feedback by Randy Stout
Everspark is a game changer: it is very, very useful for anyone doing dSTORM.
Feedback diary
Images
Features
High blinking stability over time for repeated measurements
Once mounted, the sample is stable for up to 5 weeks and can be imaged multiple times
Multicolor (incl. green)
Compatible with green, red & far red dyes (JF646, JF549, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5 and mEos2)
Ready to use
Can be used directly from the vial
Up to 6 months performance
Individualized packaging for optimal longevity
Techniques
PALM
dSTORM
HILO, TIRF pattern
Protocol
The protocol of use with a chambered coverslip
Sealing of all chambers with the lid
The protocol of use with a chambered coverslip
Sealing half of the chambers with a coverslip
The protocol of use with a glass-bottom dish
The protocol of use with a depression slide
A simplified protocol
Recipe to prepare a good sealing
Technical information

Kit description:
- 10 vials with 450 µL of Everspark buffer at 100 mM MEA in Tris.
- 1 vial per experiment.
For use with: round glass-bottom dishes, standard or chambered glass coverslips.
Lifetime:
Up to 6 months (2 months after opening the pouch + 4 months storage in the pouch).
Resources:
- Read the most Frequently Asked Questions
- Everspark Safety Datasheet
- Everspark Certificate of Analysis
Story
Esther Graudens
New projects at Idylle
"When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that's exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it".
Combining Everspark
You may also like to combine Everspark with these R&D products

Stencell
Stencell will help during the sealing step when mounting your sample

SpheroRuler
Combine Everspark with SpheroRuler and get a ready-to-use kit for dSTORM calibration