The ready-to-use dSTORM super-resolution microscopy buffer that offers long-term and stable fluorescence imaging over several weeks: pour it; seal it; image it!
Designed by Karine Monier, Arnaud Favier and Christophe PlaceReviewed by Pierre Bon, Ingrid Chamma, Benjamin Compans, Laurence Dubreil, Beatrice Durel, Tamas Gajdos, Sébastien Mailfert, Céline Malleval, Paolo Marchi, Delphine Muriaux, Jonny Nixon-Abell
Powered by the Eternity technology published in Scientific Reports
What is it for? Use it if you want someone to take care of the preparation of your blinking buffer or STED-derivative buffer, for MINFLUX-like techniques namely. Use it as well if you want to prepare samples on Monday, and image on Tuesday. Or even Friday!
Use it also if you want to prepare your samples in your lab, and image in your core facility
We prepare your buffer on demand for you. We deliver it when you need. Your buffer is ready-to-use: pour it, seal it, image it!
How to use it? Check our simplified protocol and the full protocol video
Why we chose it? Learn about the story of Everspark and us
Prices from 150€. See our offers.
High stability over time
Samples are stable over several weeks
Wide pH range
Wide pH range (5-8) where most biological phenomena occur, contrary to enzyme-based buffers that require a pH of 8 for efficient blinking.
Highly efficient dark state transition
Compatible with red and far red dSTORM blinking fluorophores (AF647, AF568, DL550, DL650)
Ready to use
Can be used directly from the vial
2 months performance
Optimal performance for 2 months after production
HILO, TIRF pattern.
Fresh batches are produced at will, every week, for each order you make. They are delivered on the spot anywhere.
The protocol in video
Everspark and us
Contact the team about their technology.
New projects at Idylle
"When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that's exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it".
Contact us about your sample preparation workflow