Use it if you want to image with red, far red and green dyes (eg. AF488). Use different colors either at the same time or one after the other.
Designed by Karine Monier, Arnaud Favier and Christophe PlaceReviewed by Pierre Bon, Ingrid Chamma, Benjamin Compans, Laurence Dubreil, Beatrice Durel, Tamas Gajdos, Sébastien Mailfert, Céline Malleval, Paolo Marchi, Delphine Muriaux, Jonny Nixon-Abell
the Eternity technology published in Scientific Reports
High stability over time for repeated measurements
One mounted sample is stable over 3 to 4 weeks and can be imaged multiple times
Multicolor (incl. green)
Compatible with green, red & far red dyes (AF488, AF647, CF647, DL550, CF568, DL650, CF680, SulfoCy5) and mEos2
Ready to use
Can be used directly from the vial
Up to 6 months performance
2 months after opening the pouch + 4 months storage in the pouch
HILO, TIRF pattern
Choose the MEA concentration, pH, optical index, or even additives
Community ongoing tests
The community is currently testing those conditions:
Multiconditions at the same time or sequentially: High Content Screening / High Troughput
The community disclosed that Everspark does not work 100% efficiently with organs and tissues because there is most of the times a bit of remaining oxygen.
And we would love to know if somebody is testing PALM + STORM at the same time, as well as multiple conditions at once using Stencell !
Everspark and us
Contact the team about their technology.
New projects at Idylle
"When I was working on long manipulations, I dreamed of being able to stop at certain crucial stages. Everspark is the ideal answer to the problem of many researchers who prepare their samples in a hurry simply because they need to image them right away. The Everspark buffer eliminates this tension: they take the time to image whenever they want, with complete peace of mind. They can also make acquisitions over several days. This will really change the way people prepare their experimentations, and that's exactly what I like about my job!
In terms of production, I am very proud of the way we have managed to set up a just-in-time production process in our labs in record time. And when researchers ask us to introduce variants, our production teams are able to build and produce very quickly their customized buffer to test it".
Contact us about your sample preparation workflow