Odoo • Image et Texte

Everspark


The ready-to-use dSTORM super-resolution microscopy buffer that offers long-term and stable fluorescence imaging over several weeks.

Powered by the Eternity technology published in Scientific Reports



    EXAMPLES OF RESULTS
     FEATURES
     PROTOCOL
     PUBLICATIONS

       

Our offers

Whether you are a buffer user who want to try Everspark in your workflow or start right away with a 15 experiments package, order what fits best your needs.

DISCOVER

150

  • 5 vials of ready-to-use buffer
  • For 5 experiments
  • Perfect to test the efficiency of Everspark
  • Option: sealing kit to optimize the trial

EVERSPARK 15

  250

  • 15 vials of ready-to-use buffer
  • For 15 experiments
  • Perfect for innovative labs
  • Option: sealing kit to optimize the first experiments

You experiment an unusual buffer reaction? We have solutions to support you. Tell us!

Designed by Karine Monier, Arnaud Favier and Christophe Place

"We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging. Indeed, an oxygen-free buffer containing a thiol reducer is central to promote fluorophore blinking and thus single molecule detection, required for dSTORM imaging. With classical buffers, such as the Glox buffer, fluorophore blinking lifetime is limited to a few hours. With Everspark buffer, it is now possible to increase sample lifetime to several weeks. We demonstrated that a calibrated sample mounted once with the Everspark buffer can be submitted to several rounds of dSTORM imaging to acquire and reconstruct a structure reproducible in coverage and precision, over a period of two months."

Odoo • Text and Image
Odoo • Image and Text
Odoo • Image and Text

  EXAMPLES OF RESULTS

Everspark is a ready-to-use dSTORM super-resolution microscopy buffer offering long-term and stable fluorescence imaging over several weeks.

Odoo • Image et Texte
Left: Long-term dSTORM imaging with Eternity
Two centrosomes imaged on a single slide, either the same Day (D0) and 7 days after mounting (D7) the sample with Eternity buffer.  The typical 450 nm donut-like structure is visualised using a colour-coded scale encoded with the  IGOR software, where each point appears as a function of its localisation precision (5 to 60 nm; inverted rainbow colour scale).
Labeling: Distal-appendages detected by immunofluorescence with AF647 in RPE-1 cells.
Right: The number of blinking events per centrosome and the median of the localisation precision in nm
Presented as a for each serie of 50,000 images recorded at D0 and D7 (left).
Credit: @Camille Fourneaux, Karine Monier - CRCL, Lyon  
Odoo • Image et Texte
Donut-like structure of in-cellulo mature centrosome reconstructed after dSTORM imaging in Eternity buffer.
Each point is represented by its centroid (purple points) and its gaussian width (white). Intensity profil is displayed on the right with the measurement from peak to peak, in agreement with the size of the distal appendage crone.

Labeling: Distal-appendages detected by immunofluorescence with AF647 in RPE-1 cells.
Credit:
@Corentin Rousset, Karine Monier, CRCL, Lyon 

                  FEATURES


                Ready to use: Everspark can be used directly from the vial. No need to add anything.

                High stability over time: Samples prepared with Everspark are stable over several weeks.

                Wide pH range: The Everspark buffer works over a wide pH range (5-8) where most biological phenomena occur, contrary to the enzyme-based buffer that requires a pH of 8 for efficient blinking.

                Efficiency: Everspark provides highly efficient dark state transition.

                Multicolor: Everspark is compatible with red and far red dSTORM blinking fluorophores (AF647, AF568, DL550, DL650).

                A performance of 2 months: The Everspark vials have proven an optimal performance for 2 months after production. 

                Applications:
                Super-resolution imaging on selected samples with the ability to observe multiple fields of views
                Statistics on multiple structures on a single sample (and not among many)
                Calibration samples based on known reference
                Ease of use for demonstration and training in super resolution imaging.

                Techniques:
                2D dSTORM
                3D dSTORM
                HILO, TIRF pattern.

                   PROTOCOL

                  PUBLICATIONS