Co-localisation studies using three-colour 3D dSTORM imaging with Everspark 2.0
Centrosomal proteins Cep152, rootletin and PCM labelled with AF647, CF568 & AF488 respectively were imaged on a Vutara VXL (Bruker) inEverspark 2.0 buffer before 3D dSTORM reconstruction
Credits: Karine Monier, INMG-PGNM, Lyon
Co-localisation studies using three-colour 3D dSTORM imaging with Everspark 2.0
Centrosomal proteins Cep152, rootletin and PCM labelled with AF647, CF568 & AF488 respectively were imaged on a Vutara VXL (Bruker) in Everspark 2.0 buffer before 3D dSTORM reconstruction
Credits: Karine Monier, INMG-PGNM, Lyon
Green-channel dSTORM imaging of AF488 fluorophore using Everspark 2.0 buffer
AF488-coated beads were mounted in Everspark 1.0 or Everspark 2.0 buffer and imaged on a Vutara VXL (Bruker) before 3D dSTORM reconstruction.
Credits: Karine Monier, INMG-PGNM, Lyon
Long-term imaging with Everspark 1.0
Left: Two centrosomes imaged the same Day (D0) and 7 days after mounting (D7) on the same slide stored in the dark at 4°C. The typical 450 nm donut-like structure is visualised using a colour-coded scale encoded with the IGOR software, where each point appears as a function of its localisation precision (5 to 60 nm; inverted rainbow colour scale). Labeling: Distal-appendages detected by immunofluorescence with AF647 in RPE-1 cells.
Right: The number of blinking events per centrosome and the median of the localization precision in nm are presented for each serie of 50,000 images recorded at D0 and D7 (left).
Credits: Camille Fourneaux & Karine Monier, CRCL, Lyon
Donut-like structure of in-cellulo mature centrosome reconstructed after dSTORM imaging in Everspark 1.0 buffer
Each point is represented by its centroid (purple points) and its gaussian width (white). Intensity profil is displayed on the right with the measurement from peak to peak, in agreement with the size of the distal appendage crone. Labeling: distal-appendages detected by immunofluorescence with AF647 in RPE-1 cells.
Credits: Corentin Rousset, Karine Monier, CRCL Lyon
Everspark is a game changer: it is very, very useful for anyone doing dSTORM.
We designed the Everspark buffer to facilitate sample preparation for dSTORM super-resolution imaging
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