Biography
I studied at the university Pierre et Marie Curie (Paris VI) and I specialized in organic chemistry. After a PhD in synthesis methodology (Pr. A. Alexakis, Paris VI) and a post-doctorate on the synthesis of the Tridemethylisovelleral, a potent cytotoxic agent (Pr. O. Sterner, Lund University, Sweden), I joined the Chemistry Department of the Ecole Normale Supérieure (Paris). I was here recruited as a research engineer and I continued research at the interface of chemistry, biology and physics in L. Jullien's group. I then started to work on the synthesis of photolabile protecting groups, caged compounds and fluorogenic ligands compatible with biological applications.Research topics
I work mostly on the synthesis, characterization and physicochemical study of chemical species with tailored spectroscopic properties.Fields of interest
Photolabile protecting groups, caged compounds, fluorescent ligands.
Technologies
Applications
Actiflash
Actiflash is the stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination
Several publications
Hamouri F, Zhang W, Aujard I, Le Saux T, Ducos B, Vriz S, Jullien L, Bensimon D. Optical control of protein activity and gene expression by photoactivation of caged cyclofen. Methods Enzymol. 2019;624:1-23. https://www.sciencedirect.com/science/article/pii/S0076687919301247Li C, Plamont MA, Sladitschek HL, Rodrigues V, Aujard I, Neveu P, Le Saux T, Jullien L, Gautier A. Dynamic multicolor protein labeling in living cells. Chem Sci. 2017 Aug 1;8(8):5598-5605. https://pubs.rsc.org/en/content/articlelanding/2017/SC/C7SC01364G
Delacour Q, Li C, Plamont MA, Billon-Denis E, Aujard I, Le Saux T, Jullien L, Gautier A. Light-Activated Proteolysis for the Spatiotemporal Control of Proteins. ACS Chem Biol. 2015 Jul 17;10(7):1643-7. https://pubs.acs.org/doi/10.1021/acschembio.5b00069
Fournier L, Aujard I, Le Saux T, Maurin S, Beaupierre S, Baudin JB, Jullien L. Coumarinylmethyl caging groups with redshifted absorption. Chemistry. 2013 Dec 16;19(51):17494-507. https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.201302630
Fournier L, Gauron C, Xu L, Aujard I, Le Saux T, Gagey-Eilstein N, Maurin S, Dubruille S, Baudin JB, Bensimon D, Volovitch M, Vriz S, Jullien L. A blue-absorbing photolabile protecting group for in vivo chromatically orthogonal photoactivation. ACS Chem Biol. 2013 Jul 19;8(7):1528-36. https://pubs.acs.org/doi/10.1021/cb400178m
Sinha DK, Neveu P, Gagey N, Aujard I, Benbrahim-Bouzidi C, Le Saux T, Rampon C, Gauron C, Goetz B, Dubruille S, Baaden M, Volovitch M, Bensimon D, Vriz S, Jullien L. Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination. Chembiochem. 2010 Mar 22;11(5):653-63. https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201000008
Gagey N, Emond M, Neveu P, Benbrahim C, Goetz B, Aujard I, Baudin JB, Jullien L. Alcohol uncaging with fluorescence reporting: evaluation of o-acetoxyphenyl methyloxazolone precursors. Org Lett. 2008 Jun 19;10(12):2341-4. https://pubs.acs.org/doi/10.1021/ol800284g
Neveu P, Aujard I, Benbrahim C, Le Saux T, Allemand JF, Vriz S, Bensimon D, Jullien L. A caged retinoic acid for one- and two-photon excitation in zebrafish embryos. Angew Chem Int Ed Engl. 2008;47(20):3744-6. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.200800037
Aujard I, Benbrahim C, Gouget M, Ruel O, Baudin JB, Neveu P, Jullien L. o-nitrobenzyl photolabile protecting groups with red-shifted absorption: syntheses and uncaging cross-sections for one- and two-photon excitation.Chemistry. 2006 Sep 6;12(26):6865-79. https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.200501393