Biography
Initially trained as a pharmacist at Université de Caen, I did a PhD in analytical and physical chemistry in the group of Prof. P. Gareil (Universite Paris 6). After a post-doc at the University of Hyogo in the group of Prof. S. Terabe, I became an assistant professor at Universite Paris 6 now Sorbonne Université in the group of Prof. Ludovic Jullien.Research topics
My research is motivated by developing and applying protocols with their associated instruments to better describe various systems ranging from a simple solution to living cells or organisms. Together with other researchers, I have introduced innovative tools and rationalized their use to selectively recover useful information or image about these systems.Fields of interest
Biophysical Chemistry, Analytical Chemistry, Thermokinetics, Photophysics.
Technologies
Applications
Actiflash
Actiflash is the stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination
Several publications
Quérard J, Zhang R, Kelemen Z, Plamont MA, Xie X, Chouket R, Roemgens I, Korepina Y, Albright S, Ipendey E, Volovitch M, Sladitschek HL, Neveu P, Gissot L, Gautier A, Faure JD, Croquette V, Le Saux T, Jullien L. Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations. Nat Commun. 2017 Oct 17;8(1):969. https://doi.org/10.1038/s41467-017-00847-3Querard J, Markus TZ, Plamont MA, Gauron C, Wang P, Espagne A, Volovitch M, Vriz S, Croquette V, Gautier A, Le Saux T, Jullien L. Photoswitching kinetics and phase-sensitive detection add discriminative dimensions for selective fluorescence imaging. Angew Chem Int Ed Engl. 2015 Feb 23;54(9):2633-7. https://doi.org/10.1002/anie.201408985
Wan P, Querard J, Maurin S, Nath S, Le Saux T, Gautier A and Jullien L. Photochemical properties of Spinach and its use in selective imaging. Chem Sci. 2013 Jan; 4(7): 2865. https://doi.org/10.1039/C3SC50729G
Emond M, Le Saux T, Allemand JF, Pelupessy P, Plasson R, Jullien L. Energy propagation through a protometabolism leading to the local emergence of singular stationary concentration profiles. Chemistry. 2012 Nov 5;18(45):14375-83. https://doi.org/10.1002/chem.201201974
Labruère R, Alouane A, Le Saux T, Aujard I, Pelupessy P, Gautier A, Dubruille S, Schmidt F, Jullien L. "Self-immolative" spacer for uncaging with fluorescence reporting. Angew Chem Int Ed Engl. 2012 Sep 10;51(37):9344-7. https://doi.org/10.1002/anie.201204032
Zrelli K, Barilero T, Cavatore E, Berthoumieux H, Le Saux T, Croquette V, Lemarchand A, Gosse C, Jullien L. Temperature modulation and quadrature detection for selective titration of two-state exchanging reactants. Anal Chem. 2011 Apr 1;83(7):2476-84. https://doi.org/10.1021/ac1026034
Barilero T, Le Saux T, Gosse C, Jullien L. Fluorescent thermometers for dual-emission-wavelength measurements: molecular engineering and application to thermal imaging in a microsystem. Anal Chem. 2009 Oct 1;81(19):7988-8000. https://doi.org/10.1021/ac901027f
Le Saux T, Chhun S, Rey E, Launay O, Weiss L, Viard JP, Pons G, Jullien V. Quantification of seven nucleoside/nucleotide reverse transcriptase inhibitors in human plasma by high-performance liquid chromatography with tandem mass-spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):81-90. https://doi.org/10.1016/j.jchromb.2008.02.008
Saux TL, Cottet H. Size-based characterization by the coupling of capillary electrophoresis to Taylor dispersion analysis. Anal Chem. 2008 Mar 1;80(5):1829-32. https://doi.org/10.1021/ac702257k
Estévez-Torres A, Le Saux T, Gosse C, Lemarchand A, Bourdoncle A, Jullien L. Fourier transform to analyse reaction-diffusion dynamics in a microsystem. Lab Chip. 2008 Jul;8(7):1205-9. https://doi.org/10.1039/B805412F