What is it for? Use it if you want to subtlely control a protein expression from a spatial and/or temporal point of view. Or if you want to perform in vivo cell tracking experiments. Use it as well if you want to easily switch on or off with light your CRE-ERT2 model.
Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Reviewed by David Bensimon, Lorenzo Bombardelli, Sidney Cambridge, Cristina Pujades, Angel Raya, Alexandre Specht, Perrine de Villemagne
Wide applicative scope
Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.
Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.
Excellent chemical stability
Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.
Favorable wavelength ranges for uncaging
Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples.
Caged Cyclofen-OH liberates Cyclofen-OH, which is photostable in contrast to Tamoxifen-OH encountering photodegradation under illumination.
Simplified protocol & conditions of success
Actiflash and us
"Once upon a time, a physicist (David Bensimon) asked a chemist (Ludovic Jullien) whether he could design a caged inducer to photocontrol protein activity in living organisms. For sure! However we also needed a biologist (Sophie Vriz) to accept the challenge to validate the caged Cyclofen-OH technology. It has been a long but so nice adventure, which has involved the tight integration of the work from many talented students, postdocs, and collaborators... Thanks to all of them!"
Contact the team about their technology
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