Actiflash

The stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination

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What is it intended for? Use it to convert your inducible ERT model into a photo-inducible one. Or if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more. 

Features and Protocol

Product information: Kit description, Safety datasheet and FAQ. 
Visit our glossary for a definition of Tamoxifen.

A list of 52 ready-to-use plasmids compatible with Actiflash.
Or you may tell us if you need a plasmid engineered model

Designed by Ludovic Jullien,Isabelle Aujard and Thomas Le Saux  
Published in ChemBioChem

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Features


Wide applicative scope


Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.


Simple conditioning


Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.


Excellent chemical stability

Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.


Favorable wavelength ranges for uncaging

Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples.

Photochemical stability

Caged Cyclofen-OH liberates Cyclofen-OH, which is photostable in contrast to Tamoxifen-OH encountering photodegradation under illumination.

Community Feedback We Found Interesting

Actiflash is efficient with zebrafish embryos of less than 48 hpf. It does not work over 48 hpf.

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