Actiflash
The stable Tamoxifen-like photoactivable inducer to perform a spatial and temporal control of your favorite proteins under illumination.
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What is it intended for?
Use it to convert your inducible ERT model into a photo-inducible one. Use it if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.
Available in a 5mg vial. Kit description, safety datasheet and FAQ
Designed by
Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Reviewed by David Bensimon, Lorenzo Bombardelli, Sidney Cambridge, Cristina Pujades, Angel Raya, Alexandre Specht, Perrine de Villemagne
Published in
ChemBioChem
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Features

Wide applicative scope
Technology capitalizing on the versatile use of Tamoxifen-OH for controlling functions of multiple types of proteins.

Simple conditioning
Caged Cyclofen-OH is cell-permeant and can be added either in the external medium or directly injected for conditioning.

Excellent chemical stability
Caged Cyclofen-OH does not generate any basal activation of protein function and it benefits from an excellent temporal resolution upon uncaging.

Favorable wavelength ranges for uncaging
Uncaging requires either UV-A light or a strong IR laser. Visible light is inactive, which facilitates the experiments with biological samples.

Photochemical stability
Caged Cyclofen-OH liberates Cyclofen-OH, which is photostable in contrast to Tamoxifen-OH encountering photodegradation under illumination.
Community Feedback We Found Interesting
Actiflash is efficient with zebrafish embryos of less than 48 hpf. It does not work over 48 hpf.
Gallery
Simplified protocol & conditions of success




Actiflash and us
"Once upon a time, a physicist (David Bensimon) asked a chemist (Ludovic Jullien) whether he could design a caged inducer to photocontrol protein activity in living organisms. For sure! However we also needed a biologist (Sophie Vriz) to accept the challenge to validate the caged Cyclofen-OH technology. It has been a long but so nice adventure, which has involved the tight integration of the work from many talented students, postdocs, and collaborators... Thanks to all of them!"
Contact the team about their technology

New projects, Idylle
Contact us if you want to discuss your illumination project . Or just share insights on a research project.