Direct STochastic Optical Reconstruction Microscopy: a type of super-resolution microscopy where a specific buffer causes the fluorophores in the sample to blink.
This blinking of the fluorophores allows algorythms to point the location of each fluorophore separately with better precision compared to having them fluoresceing all at the same time.
Then by repeating this process over a sufficient (1000+) amount of frames the algorythm may reconstruct a super-resolved image of the sample.
A range of fluorescence microscopy techniques allowing the user to go beyond the common resolution limit of conventional light microscopes (which is around 1/0,5um) down to 100 or even 10nm in resolution.
Tamoxifen is a selective estrogen receptor modulator used to prevent breast cancer in women and treat breast cancer in women and men. Its structure has inspired the design of Cyclofen, the compound used in Actiflash. Cyclofen is an analogue of Tamoxifen and is a photochemically stable inducer of the estrogen receptor specific for 4-hydroxy-tamoxifen (ERT2). At submicromolar concentrations, it activates proteins fused to the ERT2 receptor (e.g. Cre-Lox recombinase system and GAL4-UAS transcriptase system).
Useful for: Actiflash